Foil-based DNA melting curve analysis platform for low-cost point-of-care molecular diagnostics
2013 (English)In: 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, 2013, Vol. 3, 1770-1772 p.Conference paper (Refereed)
We report on genotyping of single nucleotide polymorphisms (SNP) by melting curve analysis (MCA) on DNA microarrays in a plastic microfluidic system with integrated heaters using lamination foils. Thin-film micro-heaters were processed on polyethylene napthalate (PEN) foil in only one metallization step. DNA microarrays were prepared directly on the PEN surface without the need of surface pre-treatment via UV-mediated immobilization of the spotted DNA microarrays. MCA in microfluidic channels is demonstrated at a ramping rate of 60°C/min, enabling ultra-rapid (50 seconds) SNP analysis.
Place, publisher, year, edition, pages
2013. Vol. 3, 1770-1772 p.
Lab-on-chip, Micro-heater, Microarray, Single nucleotide polymorphism
IdentifiersURN: urn:nbn:se:kth:diva-168797ScopusID: 2-s2.0-84907307461ISBN: 978-163266624-6OAI: oai:DiVA.org:kth-168797DiVA: diva2:819658
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Freiburg; Germany
QC 201506112015-06-112015-06-092015-06-11Bibliographically approved