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Modified DVD-drive as an integrated microfluidic system for precipitate-based detection of LAMP assay
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology.
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology.
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology.ORCID iD: 0000-0001-5199-0663
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2013 (English)In: 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, 2013, Vol. 3, 1616-1618 p.Conference paper, Published paper (Refereed)
Abstract [en]

Loop-mediated isothermal amplification (LAMP) is a sensitive method for nucleic acid analysis, and has been demonstrated as a ideal technique for use in miniaturized microfluidic systems. While LAMP assays are often detected using absorbance or fluorescence, we demonstrate an integrated system for LAMP assays through the detection of precipitate formation using a modified commercial DVD drive. This integrated DVD drive system is able to automate the sedimentation process and scattering-based detection of accumulated precipitate, as well as maintain the constant temperature needed for LAMP analysis.

Place, publisher, year, edition, pages
2013. Vol. 3, 1616-1618 p.
Keyword [en]
Centrifugal microfluidics, DVD imaging, LAMP, Precipitate
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:kth:diva-168794Scopus ID: 2-s2.0-84907356368OAI: oai:DiVA.org:kth-168794DiVA: diva2:819661
Conference
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Freiburg; Germany
Note

QC 20150611

Available from: 2015-06-11 Created: 2015-06-09 Last updated: 2015-06-11Bibliographically approved

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