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Dynamic behavior analysis of single cells using droplet microfluidics
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0003-3618-9944
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0001-5232-0805
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2013 (English)In: 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, 2013, Vol. 3, 1674-1676 p.Conference paper, Published paper (Refereed)
Abstract [en]

We present a droplet microfluidic platform to automatically track and characterize the behavior of single cells over time. Time series analysis of single cells was enabled by encapsulating the cells in microdroplets followed by trapping microdroplets in static array of microwells that were fabricated on chip to make the droplets addressable by their position and imaging them over time. In this paper, we demonstrate the potential of automated time-lapse imaging and image analysis approach in droplet microfluidics by studying the viability of large number of single cells over time.

Place, publisher, year, edition, pages
2013. Vol. 3, 1674-1676 p.
Keyword [en]
Automatic tracking, Cell viability, Droplet microfluidics, Fluorescent imaging, Time series analysis
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:kth:diva-168796Scopus ID: 2-s2.0-84907337604ISBN: 978-163266624-6 (print)OAI: oai:DiVA.org:kth-168796DiVA: diva2:819716
Conference
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Freiburg
Note

QC 20150611

Available from: 2015-06-11 Created: 2015-06-09 Last updated: 2015-06-11Bibliographically approved

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Authority records BETA

Periyannan Rajeswari, Prem KumarJönsson, Håkan

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Khorshidi, Mohammad AliPeriyannan Rajeswari, Prem KumarJönsson, HåkanAndersson Svahn, Helene
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Proteomics and NanobiotechnologyScience for Life Laboratory, SciLifeLab
Biological Sciences

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