Dynamic behavior analysis of single cells using droplet microfluidics
2013 (English)In: 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, 2013, Vol. 3, 1674-1676 p.Conference paper (Refereed)
We present a droplet microfluidic platform to automatically track and characterize the behavior of single cells over time. Time series analysis of single cells was enabled by encapsulating the cells in microdroplets followed by trapping microdroplets in static array of microwells that were fabricated on chip to make the droplets addressable by their position and imaging them over time. In this paper, we demonstrate the potential of automated time-lapse imaging and image analysis approach in droplet microfluidics by studying the viability of large number of single cells over time.
Place, publisher, year, edition, pages
2013. Vol. 3, 1674-1676 p.
Automatic tracking, Cell viability, Droplet microfluidics, Fluorescent imaging, Time series analysis
IdentifiersURN: urn:nbn:se:kth:diva-168796ScopusID: 2-s2.0-84907337604ISBN: 978-163266624-6OAI: oai:DiVA.org:kth-168796DiVA: diva2:819716
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Freiburg
QC 201506112015-06-112015-06-092015-06-11Bibliographically approved