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Probing bio-molecular interactions in attoliter volumes
KTH, School of Engineering Sciences (SCI), Applied Physics, Experimental Biomolecular Physics.ORCID iD: 0000-0002-1850-5440
2014 (English)In: Optical Sensors, 2014, 2014Conference paper, Published paper (Refereed)
Abstract [en]

We report on label-free volume, concentration, and mobility analysis of single protein molecules and nanoparticles during their diffusion through a subattoliter detection volume, confined by a 100 nm aperture in a thin gold film. A high concentration of small fluorescent molecules renders the aqueous solution in the aperture brightly fluorescent. Nonfluorescent analytes diffusing into the aperture displace the fluorescent molecules in the solution, leading to a decrease of the detected fluorescence signal, while analytes diffusing out of the aperture return the fluorescence level. The resulting fluorescence fluctuations provide direct information on the volume, concentration, and mobility of the nonfluorescent analytes through fluctuation analysis in both time and amplitude.

Place, publisher, year, edition, pages
2014.
Keyword [en]
Molecules, Detection volume, Fluctuation analysis, Fluorescence fluctuation, Fluorescence level, Fluorescence signals, Fluorescent molecules, Mobility analysis, Single protein molecules, Fluorescence
National Category
Biophysics
Identifiers
URN: urn:nbn:se:kth:diva-168861Scopus ID: 2-s2.0-84923901993ISBN: 9781557528209 (print)OAI: oai:DiVA.org:kth-168861DiVA: diva2:819827
Conference
Optical Sensors, 2014; Barcelona; Spain; 27 July 2014 through 31 July 2014
Note

QC 20150611

Available from: 2015-06-11 Created: 2015-06-09 Last updated: 2015-06-11Bibliographically approved

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Wennmalm, Stefan

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