Interfacing picoliter droplet microfluidics with addressable μl-compartments using FACS
2013 (English)In: 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, 2013, Vol. 3, 1632-1634 p.Conference paper (Refereed)
We present a high-throughput technique to interface picoliter droplet microfluidics for single cell analysis with a macro scale accessible array platform by the addition of an agarose gelling agent to droplets and patterned positioning of the resulting hydrogel beads using a fluorescence activated cell sorter (FACS). This resulted in a pattern with 95 % single bead accuracy. Agarose beads containing eGFP expressing E. Coli were single sorted into microwells and E. coli growth was monitored over time.
Place, publisher, year, edition, pages
2013. Vol. 3, 1632-1634 p.
Other Chemistry Topics Biological Sciences
IdentifiersURN: urn:nbn:se:kth:diva-168783ScopusID: 2-s2.0-84907360882OAI: oai:DiVA.org:kth-168783DiVA: diva2:820392
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Freiburg; Germany
FunderScience for Life Laboratory - a national resource center for high-throughput molecular bioscience
QC 201506122015-06-122015-06-092015-06-12Bibliographically approved