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Interfacing picoliter droplet microfluidics with addressable μl-compartments using FACS
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0001-5232-0805
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.
2013 (English)In: 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, 2013, Vol. 3, 1632-1634 p.Conference paper, Published paper (Refereed)
Abstract [en]

We present a high-throughput technique to interface picoliter droplet microfluidics for single cell analysis with a macro scale accessible array platform by the addition of an agarose gelling agent to droplets and patterned positioning of the resulting hydrogel beads using a fluorescence activated cell sorter (FACS). This resulted in a pattern with 95 % single bead accuracy. Agarose beads containing eGFP expressing E. Coli were single sorted into microwells and E. coli growth was monitored over time.

Place, publisher, year, edition, pages
2013. Vol. 3, 1632-1634 p.
National Category
Other Chemistry Topics Biological Sciences
Identifiers
URN: urn:nbn:se:kth:diva-168783Scopus ID: 2-s2.0-84907360882OAI: oai:DiVA.org:kth-168783DiVA: diva2:820392
Conference
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Freiburg; Germany
Funder
Science for Life Laboratory - a national resource center for high-throughput molecular bioscience
Note

QC 20150612

Available from: 2015-06-12 Created: 2015-06-09 Last updated: 2015-06-12Bibliographically approved

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Jönsson, Håkan

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Weibull, EmilieBai, YunpengJönsson, HåkanAndersson Svahn, Helen
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