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Tomographic reconstruction in soft x-ray microscopy using focus-stack back-projection
KTH, School of Engineering Sciences (SCI), Applied Physics, Biomedical and X-ray Physics.ORCID iD: 0000-0001-8604-735X
KTH, School of Engineering Sciences (SCI), Applied Physics, Biomedical and X-ray Physics.
KTH, School of Engineering Sciences (SCI), Applied Physics, Biomedical and X-ray Physics.ORCID iD: 0000-0003-2723-6622
2015 (English)In: Optics Letters, ISSN 0146-9592, E-ISSN 1539-4794, Vol. 40, no 10, 2201-2204 p.Article in journal (Refereed) Published
Abstract [en]

Tomographic reconstruction in soft x-ray microscopy is a powerful technique for obtaining high-resolution 3D images of biological samples. However, the depth of focus of such zone-plate-based microscopes is typically shorter than the thickness of many relevant biological objects, challenging the validity of the projection assumption used in conventional reconstruction algorithms. In order to make full use of the soft x-ray microscopes' high resolution, the tomographic reconstruction needs to take the depth of focus into account. Here we present a method to achieve high resolution in the full sample when the depth of focus is short compared to the sample thickness. The method relies on the back-projection of focus-stacked image data from x-ray microscopy. We demonstrate the method on theoretical and experimental data.

Place, publisher, year, edition, pages
Optical Society of America, 2015. Vol. 40, no 10, 2201-2204 p.
Keyword [en]
Image-Formation, Beamline
National Category
Other Physics Topics
URN: urn:nbn:se:kth:diva-169265DOI: 10.1364/OL.40.002201ISI: 000354708300012OAI: diva2:821664
Swedish Research Council

QC 20150615

Available from: 2015-06-15 Created: 2015-06-12 Last updated: 2016-03-24Bibliographically approved
In thesis
1. 3D X-ray microscopy: image formation, tomography and instrumentation
Open this publication in new window or tab >>3D X-ray microscopy: image formation, tomography and instrumentation
2016 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Tomography in soft X-ray microscopy is an emerging technique for obtaining quantitative 3D structural information about cells. One of its strengths, compared with other techniques, is that it can image intact cells in their near-native state at a few 10 nm’s resolution, without staining. However, the methods for reconstructing 3D-data rely on algorithms that assume projection data, which the images are generally not due to the imaging systems’ limited depth of focus. To bring out the full potential of tomography in soft X-ray microscopy an improved understanding of the image formation is desired.

This Thesis reviews zone plate-based X-ray microscopy for biological imaging and the theory necessary for a numerical implementation of a 3D image formation model. Furthermore, a novel reconstruction approach is proposed that improves the overall resolution in a reconstruction of a tomographically imaged object. This is demonstrated by simulations and experiments. Finally, this Thesis covers work on the Stockholm X-ray microscope, including an upgrade of the X-ray source yielding unprecedented brightness for a compact system. With this upgrade it was possible to do high-quality imaging of cells in their near-native state with only 10 second exposures.

Abstract [sv]

Tomografi i mjukröntgenmikroskopi är en ny teknik för att få ut kvantitativ strukturell 3D information om celler. Dess styrka jämfört med andra tekniker är att den kan avbilda intakta celler i deras nära naturliga tillstånd med ett par 10 nm upplösning, utan omfattande preparering. Dock är metoderna för att rekonstruera 3D-data beroende av algoritmer som antar projektionsdata, vilket bilderna i allmänhet inte är på grund av avbildningsystemens begränsade skärpedjup. För att få ut den fulla potentialen av tomografi i röntgenmikroskopi behövs en ökad förståelse för avbildningsprocessen.

Denna avhandling behandlar zonplatte-baserad röntgenmikroskopi för biologisk avbildning och den nödvändiga teorin för en numerisk implementering av en avbildningsmodell i 3D. En ny rekonstruktionsmetod föreslås som förbättrar upplösningen i rekonstruktionen för ett tomografiskt avbildat objekt. Detta visas i simuleringar och experiment. Slutligen omfattar denna avhandling arbete på Stockholms mjukröntgenmikroskop, inklusive en uppgradering av röntgenkällan som ger oöverträffad ljusstyrka för ett kompakt system. Denna uppgradering möjliggör högkvalitativ avbildning av celler i deras nästan naturliga tillstånd med endast 10 sekunders exponering.

Place, publisher, year, edition, pages
Stockholm: KTH Royal Institute of Technology, 2016. viii, 75 p.
TRITA-FYS, ISSN 0280-316X ; 2016:15
X-ray microscopy, image formation theory, partial coherence in imaging, wave propagation, tomography, instrumentation
National Category
Physical Sciences
Research subject
urn:nbn:se:kth:diva-184095 (URN)978-91-7595-914-6 (ISBN)
Public defence
2016-04-22, FD5, Albanova universitetscentrum, Roslagstullsbacken 21, Stockholm, 13:00 (English)
Swedish Research CouncilKnut and Alice Wallenberg Foundation

QC 20160324

Available from: 2016-03-24 Created: 2016-03-23 Last updated: 2016-03-24Bibliographically approved

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Selin, MårtenFogelqvist, EmelieHertz, Hans M.
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