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Single-cell characterization of in vitro migration and interaction dynamics of T cells expanded with IL-2 and IL-7
KTH, School of Engineering Sciences (SCI), Applied Physics. KTH, Centres, Science for Life Laboratory, SciLifeLab.
KTH, School of Engineering Sciences (SCI), Applied Physics. KTH, Centres, Science for Life Laboratory, SciLifeLab.
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2015 (English)In: Frontiers in Immunology, ISSN 1664-3224, Vol. 6, 196Article in journal (Refereed) Published
Abstract [en]

T cells are pivotal in the immune defense against cancers and infectious agents. To mount an effector response against cancer cells, T cells need to migrate to the cancer-site, engage in contacts with cancer cells, and perform their effector functions. Adoptive T cell therapy is an effective strategy as treatment of complications such as relapse or opportunistic infections after hematopoietic stem cell transplantations. This requires a sufficient amount of cells that are able to expand and respond to tumor or viral antigens. The cytokines interleukin (IL)-2 and IL-7 drive T cell differentiation, proliferation, and survival and are commonly used to expand T cells ex vivo. Here, we have used microchip-based live-cell imaging to follow the migration of individual T cells, their interactions with allogeneic monocytes, cell division, and apoptosis for extended periods of time; something that cannot be achieved by commonly used methods. Our data indicate that cells grown in IL-7 + IL-2 had similar migration and contact dynamics as cells grown in IL-2 alone. However, the addition of IL-7 decreased cell death creating a more viable cell population, which should be beneficial when preparing cells for immunotherapy.

Place, publisher, year, edition, pages
2015. Vol. 6, 196
Keyword [en]
T cell, IL-2, IL-7, microscopy, fluorescence, microchip, single-cell analysis, live-cell imaging
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URN: urn:nbn:se:kth:diva-169286DOI: 10.3389/fimmu.2015.00196ISI: 000354937600002PubMedID: 25972868OAI: diva2:822640
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Available from: 2015-06-17 Created: 2015-06-12 Last updated: 2015-06-17Bibliographically approved

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Frisk, Thomas W.Önfelt, Björn
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