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Microfluidic system for the identification of bacterial pathogens causing urinary tract infections
KTH, School of Electrical Engineering (EES), Micro and Nanosystems.ORCID iD: 0000-0002-0441-6893
KTH, School of Electrical Engineering (EES), Micro and Nanosystems.ORCID iD: 0000-0001-8248-6670
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2015 (English)In: Microfluidics, BioMEMS, and Medical Microsystems XIII, SPIE - International Society for Optical Engineering, 2015, Vol. 9320, 93200SConference paper, Published paper (Refereed)
Abstract [en]

Urinary tract infections (UTIs) are among the most common bacterial infections and pose a significant healthcare burden. The growing trend in antibiotic resistance makes it mandatory to develop diagnostic kits which allow not only the determination of a pathogen but also the antibiotic resistances. We have developed a microfluidic cartridge which takes a direct urine sample, extracts the DNA, performs an amplification using batch-PCR and flows the sample over a microarray which is printed into a microchannel for fluorescence detection. The cartridge is injection-molded out of COP and contains a set of two-component injection-molded rotary valves to switch between input and to isolate the PCR chamber during thermocycling. The hybridization probes were spotted directly onto a functionalized section of the outlet microchannel. We have been able to successfully perform PCR of E. coli in urine in this chip and perform a fluorescence detection of PCR products. An upgraded design of the cartridge contains the buffers and reagents in blisters stored on the chip.

Place, publisher, year, edition, pages
SPIE - International Society for Optical Engineering, 2015. Vol. 9320, 93200S
Series
Proceedings of SPIE, ISSN 0277-786X ; 9320
Keyword [en]
Lab-on-a-chip, Point-of-care diagnostics, microfluidics, PCR, urinary tract infections
National Category
Other Engineering and Technologies
Identifiers
URN: urn:nbn:se:kth:diva-169303DOI: 10.1117/12.2077644ISI: 000354516300020Scopus ID: 2-s2.0-84931864256ISBN: 978-1-62841-410-3 (print)OAI: oai:DiVA.org:kth-169303DiVA: diva2:822685
Conference
Conference on Microfluidics, BioMEMS, and Medical Microsystems XIII, FEB 07-09, 2015, San Francisco, CA, United States
Note

QC 20150617

Available from: 2015-06-17 Created: 2015-06-12 Last updated: 2015-09-11Bibliographically approved

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Haraldsson, Tommyvan der Wijngaart, Wouter

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