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Studies on an on/off-switchable immunosensor for troponin T
KTH, School of Biotechnology (BIO), Theoretical Chemistry and Biology. (Virtual Laboratory for Molecular Probes)ORCID iD: 0000-0003-0185-5724
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2015 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 73Article in journal (Refereed) Published
Abstract [en]

Regeneration is a key goal in the design of immunosensors. In this study, we report the temperature-regulated interaction of N-isopropylacrylamide (PNIPAAm) functionalised cardiac troponin T (cTnT) with anti-cTnT. Covalently bonded PNIPAAm on an anti-cTnT bioelectrode showed on/off-switchability, regeneration capacity and temperature triggered sensitivity for cTnT. Above the lower critical solution temperature (LCST), PNIPAAm provides a liphophilic microenvironment with specific volume reduction at the bioelectrode surface, making available binding space for cTnT, and facilitating analyte recognition. Computational studies provide details about the structural changes occurring at the electrode above and below the LCST. Furthermore, free energies associated with the binding of cTnT with PNIPAAm at 25 (δG<inf>coil</inf>=-6.0Kcal/mole) and 37°C (δG<inf>globular</inf>=-41.0kcal/mole) were calculated to elucidate the interaction and stability of the antigen-antibody complex. The responsiveness of such assemblies opens the way for miniaturised, smart immuno-technologies with 'built-in' programmable interactions of antigen-antibody upon receiving stimuli.

Place, publisher, year, edition, pages
2015. Vol. 73
Keyword [en]
On/off-switching, Programmable bioelectronics, Smart immunosensors, Troponin T
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:kth:diva-170187DOI: 10.1016/j.bios.2015.05.055ISI: 000358823900014Scopus ID: 2-s2.0-84930629687OAI: oai:DiVA.org:kth-170187DiVA: diva2:828288
Funder
Swedish Institute, 00037/2014
Note

QC 20150630

Available from: 2015-06-30 Created: 2015-06-29 Last updated: 2017-12-04Bibliographically approved

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Arul Murugan, Natarajan

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