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Lactoperoxidase-mediated degradation of single-walled carbon nanotubes in the presence of pulmonary surfactant
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2015 (English)In: Carbon, ISSN 0008-6223, E-ISSN 1873-3891, Vol. 91, 506-517 p.Article in journal (Refereed) Published
Abstract [en]

Carbon nanotubes (CNTs) may elicit inflammatory responses following pulmonary exposure. Conversely, enzymatic biodegradation of CNTs by inflammatory cells has also been reported. The aim of this study was to study the degradation of oxidized single-walled CNTs (ox-SWCNTs) by lactoperoxidase (LPO), a secreted peroxidase present in the airways, and whether pulmonary surfactant affects this biodegradation. To this end, ox-SWCNTs were incubated in vitro with recombinant bovine LPO + H<inf>2</inf>O<inf>2</inf> + NaSCN in the presence and absence of porcine lung surfactant (Curosurf®) and biodegradation was monitored using UV-Vis-NIR spectroscopy, Raman spectroscopy, and scanning electron microscopy. The interaction of recombinant LPO with bundles of ox-SWCNTs was confirmed by atomic force microscopy. Cell-free biodegradation of ox-SWCNTs was also observed ex vivo in murine bronchoalveolar lavage fluid in the presence of H<inf>2</inf>O<inf>2</inf> + NaSCN. Our study provides evidence for biodegradation of ox-SWCNTs with a lung surfactant 'bio-corona' and expands the repertoire of mammalian peroxidases capable of biodegradation of ox-SWCNTs. These findings are relevant to inhalation exposure to these materials, as LPO serves as an important component of the airway defense system.

Place, publisher, year, edition, pages
2015. Vol. 91, 506-517 p.
Keyword [en]
Atomic force microscopy, Biodegradation, Biological organs, Carbon, Mammals, Microbiology, Near infrared spectroscopy, Phospholipids, Scanning electron microscopy, Surface active agents, Yarn, Bronchoalveolar lavage fluid, Inflammatory cells, Inflammatory response, Inhalation exposure, Lactoperoxidase, Lung surfactants, Mammalian peroxidase, Pulmonary surfactants
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Other Engineering and Technologies
URN: urn:nbn:se:kth:diva-170240DOI: 10.1016/j.carbon.2015.05.022ScopusID: 2-s2.0-84930210426OAI: diva2:828457

QC 20150630

Available from: 2015-06-30 Created: 2015-06-29 Last updated: 2015-06-30Bibliographically approved

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Zhao, YinchenToprak, Muhammet Sadaka
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