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The History of Pyrosequencing®
KTH, School of Biotechnology (BIO), Industrial Biotechnology.
2015 (English)In: Methods in molecular biology (Clifton, N.J.), Vol. 1315, 3-15 p.Article in journal (Refereed) Published
Abstract [en]

One late afternoon in the beginning of January 1986, bicycling from the lab over the hill to the small village of Fulbourn, the idea for an alternative DNA sequencing technique came to my mind. The basic concept was to follow the activity of DNA polymerase during nucleotide incorporation into a DNA strand by analyzing the pyrophosphate released during the process. Today, the technique is used in multidisciplinary fields in academic, clinical, and industrial settings all over the word. This technique can be used for both single-base sequencing and whole-genome sequencing, depending on the format used.In this chapter, I give my personal account of the development of Pyrosequencing()-beginning on a winter day in 1986, when I first envisioned the method-until today, nearly 30 years later.

Place, publisher, year, edition, pages
2015. Vol. 1315, 3-15 p.
National Category
Medical Biotechnology
URN: urn:nbn:se:kth:diva-170708DOI: 10.1007/978-1-4939-2715-9_1PubMedID: 26103887ScopusID: 2-s2.0-84933060152OAI: diva2:840112

QC 20150707

Available from: 2015-07-07 Created: 2015-07-03 Last updated: 2015-10-05Bibliographically approved

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