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Optical detection of two-color-fluorophore barcode for nanopore DNA sensing
KTH, School of Information and Communication Technology (ICT), Materials- and Nano Physics, Material Physics, MF.ORCID iD: 0000-0002-8962-1844
KTH, School of Information and Communication Technology (ICT), Materials- and Nano Physics, Material Physics, MF.ORCID iD: 0000-0003-2562-0540
KTH, School of Information and Communication Technology (ICT), Materials- and Nano Physics, Material Physics, MF.ORCID iD: 0000-0002-5260-5322
2015 (English)Conference paper, Published paper (Refereed)
Abstract [en]

A simple schematic on parallel optical detection of two-fluorophore barcode for single-molecule nanopore sensing is presented. The chosen two fluorophores, ATTO-532 and DY-521-XL, emitting in well-separated spectrum range can be excited at the same wavelength. A beam splitter was employed to separate signals from the two fluorophores and guide them to the same CCD camera. Based on a conventional microscope, sources of background in the nanopore sensing system, including membranes, compounds in buffer solution, and a detection cell was characterized. By photoluminescence excitation measurements, it turned out that silicon membrane has a negligible photoluminescence under the examined excitation from 440 nm to 560 nm, in comparison with a silicon nitrite membrane. Further, background signals from the detection cell were suppressed. Brownian motion of 450 bps DNA labelled with single ATTO-532 or DY-521-XL was successfully recorded by our optical system.

Place, publisher, year, edition, pages
2015. Vol. 9519, 95190G
Series
Proceedings of SPIE, ISSN 0277-786X
National Category
Nano Technology Other Physics Topics
Identifiers
URN: urn:nbn:se:kth:diva-171918DOI: 10.1117/12.2179626ISI: 000357932000004ISBN: 978-1-62841-642-8 (print)OAI: oai:DiVA.org:kth-171918DiVA: diva2:845304
Conference
Conference on Nanotechnology VII, MAY 04-06, 2015, Barcelona, SPAIN
Note

QC 20150811

Available from: 2015-08-11 Created: 2015-08-10 Last updated: 2015-08-11Bibliographically approved

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Sychugov, IlyaLinnros, Jan

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