Effekter av lågdosbestrålning vid p53-medierad celldöd
Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesisAlternative title
Response of p53 mediated cell death to low dose irradiation (English)
The aim of the study was to investigate if PRIMA-1, a drug that reactivate the tumour suppressor gene p53, will have a synergistic effect when used in combination with γ-irradiation in a human small cell lung cancer cell line (U-1690), which show a low-dose hypersensitivity in clonogenic survival experiments. Clonogenic cell survival after incubation with PRIMA-1 alone in concentrations from 0.1 to 30 µM or in combination with γ-irradiation with a 137Cs source was performed. The standard LQ model, and the repairable-conditionally repairable damage model, the RCR model, were fitted to the experimental data. PRIMA-1 showed a time and concentration dependent cytotoxic, and 10 µM killed 60 % of cells during 72 hours incubation. The cells show low-dose hypersensitivity calculated with the RCR model. The combined treatment with 8 µM PRIMA-1 had a synergistic effect with irradiation doses from 2 Gy and above. The overall conclusion is that a combination with PRIMA-1 and irradiation is beneficial to achieve the best effect.
Place, publisher, year, edition, pages
2015. , 42 p.
PRIMA-1, 137Cs, p53, synergy, hypersensitivity
Other Physics Topics
IdentifiersURN: urn:nbn:se:kth:diva-172448OAI: oai:DiVA.org:kth-172448DiVA: diva2:848284