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Utveckling av sandwichimmunoanalyser för detektering av muskeldystrofi biomarkörer
KTH, School of Biotechnology (BIO).
2015 (Swedish)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesisAlternative title
Development of sandwich immunoassays for detection of muscular dystrophy biomarkers (English)
Abstract [en]

Due to existing limitations of diagnosis and clinical management of muscular dystrophies there is an urgent need a clinical assay which is capable of diagnosis, monitoring disease progression and severity and individual patients' responses to treatment outcome. An immunoassay requires minute amount of sample and is simpler and less expensive than today's clinical tests, which is a tremendous advantage when handling rare diseases since both the sample cohorts and the volumes are limited. This is addressed by developing a microspere-based sandwich immunoassay targeting biomarkers for Duchenne muscular dystrophy, which has been assessed in previous reports. four protein biomarkers have been target in this study; Carbonic anhydrase 3 (CA3), Malate dehydrogenase 2 (MDH2), Electron transfer flavoprotein a (ETFA) and Myosin light chain 3 (MYL3). Antibodies against these proteins have been evaluated, their target binding capacity first in a single-binder assay and eventual in a dual-binder assay have been tested, using target spike-in samples and patient samples. One prominent antibody pair targeting CA3 was developed. Antibody pairs targetin MDH2 and ETFA generates signal in patients sample although optimization is needed. These sandwich immunoassay hold great promise in the managmenet of the challenging DMD disease., by facilitating diagnosis and the monitoring of the disease progressiona dn is capable of patient stratification. The immunoassays can progress to a clinical test that offers a robust and easily assessed severity biomarker detection system.

Place, publisher, year, edition, pages
Keyword [en]
Proteomics, antibodies, suspension bead arrays, sandwich immunoassays, protein biomarkers, Buchenne muscular dystrophy
National Category
Engineering and Technology
URN: urn:nbn:se:kth:diva-173111OAI: diva2:851710
Available from: 2015-09-07 Created: 2015-09-07 Last updated: 2015-09-07Bibliographically approved

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