Hexosomes as Drug Delivery Vehicles for Antimicrobial Peptides
Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
This master thesis project was carried out at SP Technical Research Institute of Sweden within the FORMAMP project which goal is to increase the efficiency and stability of antimicrobial peptides (AMPs) by exploring and developing a number of innovative formulation strategies for the drug delivery of those systems. In view of the growing problem of bacterial resistance to traditional antibiotics, AMPs represent one of the most promising alternatives as therapeutics against infectious diseases: besides having a fast and non-specific mechanism of action, they are less prone to bacterial resistance.
In this project, the goal was to develop an efficient method for the formulation of hexagonal lyotropic phase nanodispersions (called hexosomes) as drug delivery vehicles for the AP114, DPK-060 and LL-37 AMPs. Then, these formulations were characterized through size measurements, zeta potential measurements, SAXS, cryo-TEM and UPLC and their stability was assessed. Lastly, the interaction of these systems with model bacterial membranes was tested through QCM-D and ellipsometry.
The relevant samples were found to have a hexagonal structure with the lattice parameter being larger when peptide was loaded. The systems were observed to be sufficiently stable and the peptide loading efficiency was found to be higher than 90% in most cases. The hexosomes loaded with LL-37 were observed to preserve the effectiveness of the peptide when interacting with the model membrane through QCM-D.
Place, publisher, year, edition, pages
2015. , 83 p.
hexosome, peptide, antimicrobial, QCM, bacterial
IdentifiersURN: urn:nbn:se:kth:diva-172360OAI: oai:DiVA.org:kth-172360DiVA: diva2:852802