Fluorescence labeled microbubbles for multimodal imaging
2015 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 464, no 3, 737-742 p.Article in journal (Refereed) Published
Air-filled polyvinyl alcohol microbubbles (PVA-MBs) were recently introduced as a contrast agent for ultrasound imaging. In the present study, we explore the possibility of extending their application in multimodal imaging by labeling them with a near infrared (NIR) fluorophore, VivoTag-680. PVA-MBs were injected intravenously into FVB/N female mice and their dynamic biodistribution over 24 h was determined by 3D-fluorescence imaging co-registered with 3D-mu CT imaging, to verify the anatomic location. To further confirm the biodistribution results from in vivo imaging, organs were removed and examined histologically using bright field and fluorescence microscopy. Fluorescence imaging detected PVA-MB accumulation in the lungs within the first 30 min post-injection. Redistribution to a low extent was observed in liver and kidneys at 4 h, and to a high extent mainly in the liver and spleen at 24 h. Histology confirmed PVA-MB localization in lung capillaries and macrophages. In the liver, they were associated with Kupffer cells; in the spleen, they were located mostly within the marginal-zone. Occasional MBs were observed in the kidney glomeruli and interstitium. The potential application of PVA-MBs as a contrast agent was also studied using ultrasound (US) imaging in subcutaneous and orthotopic pancreatic cancer mouse models, to visualize blood flow within the tumor mass. In conclusion, this study showed that PVA-MBs are useful as a contrast agent for multimodal imaging. (C) 2015 Elsevier Inc. All rights reserved.
Place, publisher, year, edition, pages
2015. Vol. 464, no 3, 737-742 p.
In vivo imaging systems, Micro-computed tomography, Micro-ultrasound, Microbubbles, VivoTag 680, Near infrared (NIR), Fluorescence
Biochemistry and Molecular Biology Biophysics
IdentifiersURN: urn:nbn:se:kth:diva-173428DOI: 10.1016/j.bbrc.2015.07.017ISI: 000359881900010PubMedID: 26187672ScopusID: 2-s2.0-84938738927OAI: oai:DiVA.org:kth-173428DiVA: diva2:853681
QC 201509142015-09-142015-09-112015-09-14Bibliographically approved