C-13 SPE MAS measurement of ligand concentration in compressible chromatographic beads
2015 (English)In: Magnetic Resonance in Chemistry, ISSN 0749-1581, E-ISSN 1097-458X, Vol. 53, no 8, 572-577 p.Article in journal (Refereed) Published
A method for measuring the ligand concentration in heterogeneous materials like chromatography media is described. In this method, C-13 single pulse excitation magic angle spinning NMR experiment with broadband H-1 decoupling is used to determine the peak integrals for a butyl ligand in the spectrum of a dried chromatography medium. Within a carefully controlled protocol, those integrals compared with that of the internal reference compound dimethyl sulfone provide the required volume concentration with an accuracy of ca 2%. The effects of temperature, degree of hydration, and other experimental parameters are discussed. Copyright (C) 2015 The Authors. Magnetic Resonance in Chemistry published by John Wiley & Sons Ltd.
Place, publisher, year, edition, pages
2015. Vol. 53, no 8, 572-577 p.
NMR, C-13, solid-state NMR, quantitative NMR, agarose, pore
IdentifiersURN: urn:nbn:se:kth:diva-173449DOI: 10.1002/mrc.4257ISI: 000359802300003PubMedID: 26053054ScopusID: 2-s2.0-84946492473OAI: oai:DiVA.org:kth-173449DiVA: diva2:853773
FunderSwedish Research Council
QC 201509152015-09-152015-09-112015-09-15Bibliographically approved