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Bakteriellt transmembranglykosyltransferas XCGT
KTH, School of Biotechnology (BIO).
2014 (Swedish)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesisAlternative title
Bacterial transmembrane glycosyltransferas XCGT (Swedish)
Abstract [en]

Glycosyltransferases (GT) are enzymes that synthesise vital complexes consisting of one or more sugar molecule units. A large number of GTs are integral membrane proteins. This means they can be found embedded in the many different membrane structures of cells. One example is the bacterial glycosyltransferase XCGT.

Few threee-dimensional structures have been solved for membrane GTs because of the technical difficultires that arie in work with integral membrane proteins. As a result there is only limited information available on the interplay of structure and function for membrane GTs. The aim of thisproject is to characterise XCGT biochemically and optimise conditions for increased protein stability and well.ordered, crystallisation. This should pave the road to a successfully determined crystalstructure. This thesis reports the project before publication in a peer-reviewed journal and for this reason some information is not disclosed. Yet, specific protein characteristics and methodological details are presented and supported by theory and experimental data.

The thesis opens with a theoretical review of the family of glycosyltransferases and methodology for work with membrane proteins. In addition, a background is provided for later discussion of the effect of certaindetergents on protein stability. Choice of detergent, additives and crystallisation format are shown to have critical influence on crystallisation. An outlook on alternative techniques that may improve the chances to solve XCGT's crystal structure forms the closing of the thesis.

Throughout the project, methods have been refined and results were obtained that will havesignificant importance for the continued efforts to determine the crystal structure of XGCT, and themethodological findings can even be extrapolated to the work with membrane proteins in general.

Place, publisher, year, edition, pages
Keyword [en]
transmembrane protein, detergent optimization, monodispersity, crystallization, glycosyltransferase
National Category
Engineering and Technology
URN: urn:nbn:se:kth:diva-173626OAI: diva2:854019
Available from: 2015-09-15 Created: 2015-09-15 Last updated: 2015-09-15Bibliographically approved

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