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Development of microdevices for digital ELISA
KTH, School of Biotechnology (BIO).
2014 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Immunoassays are widely used for the detection and quantification of proteins in medical diagnostics. In Medicine, sensitive and specific detection of a causative agent is crucial concerning early diagnosis of diseases and monitoring the disease status. Microfluidics platform in combination with immunoassay has beome demanding for point of care diagnostics as it provides faster, high throughput analysis with higher sensitivity and lowered cost per analysis. Recently, sensitivity has been enhanced by single molecule immunoassays also called digital ELISA, enable detection of low abundance proteins based on single molecule counting.

In this thesis work, micro devices were rabricated using UV cross-linking polymer for digital ELISA application. The digital ELISA was first developed in the bead based format. Bead based assay was developed by using streptavidin coated magnetic beads and protein analytes were capture. For detection, dual labeled gold nanoparticles aiming colorimetric detection through gold silver enhancement were used. LOD of IL-2 antigen concentration was demonstrated 0.008ug/ml. Micro devices consisting of array of microwells were fabricated by using a UV cross-linkable polymer. The developed bead based ELISA was integrated into digital ELISA format.

Place, publisher, year, edition, pages
Keyword [en]
microfluidics, ELISA, OSTE, microwells
National Category
Engineering and Technology
URN: urn:nbn:se:kth:diva-173630OAI: diva2:854047
Available from: 2015-09-15 Created: 2015-09-15 Last updated: 2015-09-15Bibliographically approved

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