Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Toward libraries for increased bio plastic production in cyanobacteria
KTH, School of Biotechnology (BIO).
2014 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesisAlternative title
Metagenomiska bibliotek att förbättra cyanobakterier bioplast produktion (Swedish)
Abstract [en]

Cyanobateria are promising cell factories due to their minimal nutrient requirements and utilization of asmospheric carbon di-oxide as its sole carbon source. In particular, polyhydroxybutyrate (PHB) is an industrially useful bio plastic that is produced naturally by some cyanobacteria. Furthermore, PHB biosynthetic pathway is a starting point for production of the biofuel, 1-butanol. There has been much genetic engineering effort toward increasing the production of PHB from cyanobacteria. These have been focused on increasing the pool of acetyl-CoA precursor, or increasing the amount of the reductant NADPH. The upstream process for increasing these reactants is complex and involves many genes. In this contect, cyanobacteria libraries will contribute to reveal genes or gene fragments that are responsible for production of PHB, alkanes and other high value compounds. In pursuit of finding these novel genes or genefragments, a transcription factor library is created in this study with 50 transcription factors. Furthermore, the process is optimized towards the creation of genomic fragment library and metagenomic fragment library with 26 diverse strains. Membersof the transcription factor library are over-expressed by a PHB - producing host Synechocystis PCC 6803 and the process towards creation of genomic and metagenomic libraries is optimized. The members of the metagenomic library can be screened for increased PHB, alkanes, lactate and other high value products and the potential members can be isolated and characterized.  

Place, publisher, year, edition, pages
2014.
Keyword [en]
cyanobacteria, metagenomics, libraries, bioplastic, transcription factors
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:kth:diva-173649OAI: oai:DiVA.org:kth-173649DiVA: diva2:854226
Supervisors
Examiners
Available from: 2015-09-18 Created: 2015-09-16 Last updated: 2017-01-11Bibliographically approved

Open Access in DiVA

fulltext(2370 kB)46 downloads
File information
File name FULLTEXT01.pdfFile size 2370 kBChecksum SHA-512
e577897e2f10be523e7499970005a9d9983e6049ec4fc82dce1c690c382572cc453fb127de29a4eb596203c3d731f1ee36485c19c0555ec206ff50061b9256dd
Type fulltextMimetype application/pdf

By organisation
School of Biotechnology (BIO)
Engineering and Technology

Search outside of DiVA

GoogleGoogle Scholar
Total: 46 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

urn-nbn

Altmetric score

urn-nbn
Total: 126 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf