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Regulating the production of (R)-3-hydroxybutyrate in Escherichia coil by N or P limitation
KTH, School of Biotechnology (BIO), Industrial Biotechnology. Univ Mayor de San Simon, Fac Sci & Technol, Ctr Biotechnol.
KTH, School of Biotechnology (BIO), Industrial Biotechnology.ORCID iD: 0000-0002-7916-4731
KTH, School of Biotechnology (BIO), Industrial Biotechnology.
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2015 (English)In: Frontiers in Microbiology, ISSN 1664-302X, E-ISSN 1664-302X, Vol. 6, 844Article in journal (Refereed) Published
Abstract [en]

The chiral compound (R)-3-hydroxybutyrate (3HB) is naturally produced by many wild type organisms as the monomer for polyhydroxybutyrate (PHB). Both compounds are commercially valuable and co-polymeric polyhydroxyalkanoates have been used e.g., in medical applications for skin grafting and as components in pharmaceuticals. In this paper we investigate cultivation strategies for production of 3HB in the previously described E. coil strain AF1000 pJBGT3RX. This strain produces extracellular 3HB by expression of two genes from the PHB pathway of Halomonas boliviensis. H. boliviensis is a newly isolated halophile that forms PHB as a storage compound during carbon excess and simultaneous limitation of another nutrient like nitrogen and phosphorous. We hypothesize that a similar approach can be used to control the flux from acetylCoA to 3HB also in E coli; decreasing the flux to biomass and favoring the pathway to the product. We employed ammonium- or phosphate-limited fed-batch processes for comparison of the productivity at different nutrient limitation or starvation conditions. The feed rate was shown to affect the rate of glucose consumption, respiration, 3HB, and acetic acid production, although the proportions between them were more difficult to affect. The highest 3HB volumetric productivity, 1.5 g L-1 h(-1), was seen for phosphate-limitation.

Place, publisher, year, edition, pages
2015. Vol. 6, 844
Keyword [en]
E. coil, 3-hydroxybutyrate (3HB), polyhydroxybutyrate (PHB), fed-batch, phosphate, ammonium, limitation, depletion
National Category
Microbiology
Identifiers
URN: urn:nbn:se:kth:diva-173438DOI: 10.3389/fmicb.2015.00844ISI: 000360116800001OAI: oai:DiVA.org:kth-173438DiVA: diva2:855139
Funder
Swedish Research Council FormasSida - Swedish International Development Cooperation Agency
Note

QC 20150918

Available from: 2015-09-18 Created: 2015-09-11 Last updated: 2017-12-05Bibliographically approved

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