Transcriptome engineering for improved industrial phenotypes in cyanobacteria
Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Industrial production of biofuels using microorganisms is facing many challenges. Among these low stress toerlance, low growth rate and low productivity are found. To address these problems transcriptome engineering is a method of increasing interest. In this study transcriptome engineering was employed to find ways to create industrial strains of the cyanobacteria Synechocystis sp. PCC6803 and Synechococcus elongatus PCC7942. Transcription factors know to be involved in stress response or in carbon fixating regulatory mechanisms in Synechocystis sp. PCC6803 were overexpressed, deleted or mutated to evaluate their effect of improving solvent tolerance in particular, but also tolerance to high temperature and oxidative stress, or of improving the growth rate and partitioning carbon fluxes towards biofuels. A 2-3 fold increase in heat tolerance of strains overexpressing Hik34 or SigB and a Hik34 knock out strain was found, a library of Ycf30 of 10 000 members with 4-7 mutations per gene was created, cultivation conditions facilitating growth of large number of cultures were established and methods for assessing butanol tolerance was determined.
Place, publisher, year, edition, pages
Engineering and Technology
IdentifiersURN: urn:nbn:se:kth:diva-173900OAI: oai:DiVA.org:kth-173900DiVA: diva2:855806