Investigation of factors affecting the enzymatic production of DNA oligonucleotides
Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
As the researches and dvelopments in DNA nanotechnology is increasing steadily, the demands for the research tools associated also started to rise gradually. To make CNA nanotechnology a viable technology for therapeutics, diagnostics or life science research, the above requirements have to be met and the component materials should be made affordable. There is a high need for producing single stranded DNA cheaply, abundantly and with very high quality. Prior experiments demonstrated the produciton of clonal template through a highly scalable enzymatic process called Monoclonal StoichiometrIC (MOSIC) method for producing such single stranded DNA oligonucleotides perfectly in a controlled relative stoichiometric ratio. This MOSIC mehtod utilizes the helper phages and bacteria to produce ssDNA in large quantity very cheaply which is highly appreciable. But after the revovery of ssDNA from phages, when they are cut with restriction enzymes to release the DNA oligonucleotides, some contaminations were seen in the digested mixture die to ineffective digestion. The aim of this project is to investigate the factors affecting the production of oligonucleotides by amplification of phagemid vector via helper phages and to find out the reason for the ineffective digestion.
Place, publisher, year, edition, pages
Engineering and Technology
IdentifiersURN: urn:nbn:se:kth:diva-173901OAI: oai:DiVA.org:kth-173901DiVA: diva2:855825