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Two-Dimensional Crystallization Procedure, from Protein Expression to Sample Preparation
KTH, School of Technology and Health (STH), Basic Science and Biomedicine, Structural Biotechnology. Karolinska Institutet, Sweden.
KTH, School of Technology and Health (STH), Basic Science and Biomedicine, Structural Biotechnology. Karolinska Institutet, Sweden.ORCID iD: 0000-0002-3220-9402
2015 (English)In: BioMed Research International, ISSN 2314-6133, E-ISSN 2314-6141, 693869Article, review/survey (Refereed) Published
Abstract [en]

Membrane proteins play important roles for living cells. Structural studies of membrane proteins provide deeper understanding of their mechanisms and further aid in drug design. As compared to other methods, electron microscopy is uniquely suitable for analysis of a broad range of specimens, from small proteins to large complexes. Of various electron microscopic methods, electron crystallography is particularly well-suited to study membrane proteins which are reconstituted into two-dimensional crystals in lipid environments. In this review, we discuss the steps and parameters for obtaining large and well-ordered twodimensional crystals. A general description of the principle in each step is provided since this information can also be applied to other biochemical and biophysical methods. The examples are taken from our own studies and published results with related proteins. Our purpose is to give readers a more general idea of electron crystallography and to share our experiences in obtaining suitable crystals for data collection.

Place, publisher, year, edition, pages
2015. 693869
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:kth:diva-173997DOI: 10.1155/2015/693869ISI: 000360748700001Scopus ID: 2-s2.0-84941130820OAI: oai:DiVA.org:kth-173997DiVA: diva2:858149
Funder
Swedish Research Council
Note

QC 20151001

Available from: 2015-10-01 Created: 2015-09-24 Last updated: 2017-12-01Bibliographically approved

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Hebert, Hans

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