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A disposable sampling device to collect volume-measured DBS directly from a fingerprick onto DBS paper
KTH, School of Electrical Engineering (EES), Micro and Nanosystems.
KTH, School of Electrical Engineering (EES), Micro and Nanosystems.ORCID iD: 0000-0001-9552-4234
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2015 (English)In: Bioanalysis, ISSN 1757-6180, E-ISSN 1757-6199, Vol. 7, no 16, 2085-2094 p.Article in journal (Refereed) Published
Abstract [en]

Background: DBS samples collected from a fingerprick typically vary in volume and homogeneity and hence make an accurate quantitative analysis of DBS samples difficult. Results: We report a prototype which first defines a precise liquid volume and subsequently stores it to a conventional DBS matrix. Liquid volumes of 2.2 mu l +/- 7.1% (n = 21) for deionized water and 6.1 mu l +/- 8.8% (n = 15) for whole blood have been successfully metered and stored in DBS paper. Conclusion: The new method of collecting a defined volume of blood by DBS sampling has the potential to reduce assay bias for the quantitative evaluation of DBS samples while maintaining the simplicity of conventional DBS sampling.

Place, publisher, year, edition, pages
2015. Vol. 7, no 16, 2085-2094 p.
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:kth:diva-173996DOI: 10.4155/bio.15.134ISI: 000360713600013PubMedID: 26327187Scopus ID: 2-s2.0-84940738836OAI: oai:DiVA.org:kth-173996DiVA: diva2:858183
Funder
Stockholm County Council, 20130765EU, European Research Council, 267528
Note

QC 20151001

Available from: 2015-10-01 Created: 2015-09-24 Last updated: 2017-12-01Bibliographically approved

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Stemme, Göran

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