Genome-wide mapping of promoter-anchored interactions with close to single-enhancer resolution
2015 (English)In: Genome Biology, ISSN 1465-6906, E-ISSN 1474-760X, Vol. 16, 156Article in journal (Refereed) Published
Although the locations of promoters and enhancers have been identified in several cell types, we still have limited information on their connectivity. We developed HiCap, which combines a 4-cutter restriction enzyme Hi-C with sequence capture of promoter regions. Applying the method to mouse embryonic stem cells, we identified promoter-anchored interactions involving 15,905 promoters and 71,984 distal regions. The distal regions were enriched for enhancer marks and transcription, and had a mean fragment size of only 699 bp - close to single-enhancer resolution. High-resolution maps of promoter-anchored interactions with HiCap will be important for detailed characterizations of chromatin interaction landscapes.
Place, publisher, year, edition, pages
2015. Vol. 16, 156
IdentifiersURN: urn:nbn:se:kth:diva-173984DOI: 10.1186/s13059-015-0727-9ISI: 000360682300001PubMedID: 26313521ScopusID: 2-s2.0-84940034154OAI: oai:DiVA.org:kth-173984DiVA: diva2:858850
FunderSwedish Foundation for Strategic Research , FFL4Swedish Research CouncilEU, European Research Council, 243066Wenner-Gren Foundations
QC 201510052015-10-052015-09-242015-10-28Bibliographically approved