Change search
ReferencesLink to record
Permanent link

Direct link
Peptide Anchor for Folate-Targeted Liposomal Delivery
Show others and affiliations
2015 (English)In: Biomacromolecules, ISSN 1525-7797, E-ISSN 1526-4602, Vol. 16, no 9, 2904-2910 p.Article in journal (Refereed) Published
Abstract [en]

Specific folate receptors are abundantly overexpressed in chronically activated macrophages and in most cancer cells. Directed folate receptor targeting using liposomes is usually achieved using folate linked to a phospholipid or cholesterol anchor. This link is formed using a large spacer like polyethylene glycol. Here, we report an innovative strategy for targeted liposome delivery that uses a hydrophobic fragment of surfactant protein D linked to folate. Our proposed spacer is a small 4 amino acid residue linker. The peptide conjugate inserts deeply into the lipid bilayer without affecting liposomal integrity, with high stability and specificity. To compare the drug delivery potential of both liposomal targeting systems, we encapsulated the nuclear dye Hoechst 34580. The eventual increase in blue fluorescence would only be detectable upon liposome disruption, leading to specific binding of this dye to DNA. Our delivery system was proven to be more efficient (2-fold) in Caco-2 cells than classic systems where the folate moiety is linked to liposomes by polyethylene glycol.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2015. Vol. 16, no 9, 2904-2910 p.
Keyword [en]
Surfactant Protein-D, Cyclooxygenase-2 Inhibitor, Inflammatory Response, Carbon-Monoxide, In-Vitro, Celecoxib, Cancer, Cells, Methotrexate, Mechanisms
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy) Biochemistry and Molecular Biology Cell Biology
URN: urn:nbn:se:kth:diva-174926DOI: 10.1021/acs.biomac.5b00823ISI: 000361341700037PubMedID: 26241560ScopusID: 2-s2.0-84941584525OAI: diva2:865268

QC 20151027

Available from: 2015-10-27 Created: 2015-10-09 Last updated: 2015-10-27Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMedScopus

Search in DiVA

By author/editor
Härmark, JohanMoreira, Alexandra
By organisation
Structural Biotechnology
In the same journal
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)Biochemistry and Molecular BiologyCell Biology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 41 hits
ReferencesLink to record
Permanent link

Direct link