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Copper-based nanoparticles induce high toxicity in leukemic HL60 cells
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Surface and Corrosion Science.ORCID iD: 0000-0002-5263-6487
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Surface and Corrosion Science.ORCID iD: 0000-0003-2206-0082
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2015 (English)In: Toxicology in Vitro, ISSN 0887-2333, E-ISSN 1879-3177, Vol. 29, no 7, 1711-1719 p.Article in journal (Refereed) Published
Abstract [en]

From the increasing societal use of nanoparticles (NPs) follows the necessity to understand their potential toxic effects. This requires an in-depth understanding of the relationship between their physicochemical properties and their toxicological behavior. The aim of the present work was to study the toxicity of Cu and CuO NPs toward the leukemic cell line HL60. The toxicity was explored in terms of mitochondrial damage, DNA damage, oxidative DNA damage, cell death and reactive oxygen species (ROS) formation. Particle characteristics and copper release were specifically investigated in order to gain an improved understanding of prevailing toxic mechanisms. The Cu NPs revealed higher toxicity compared with both CuO NPs and dissolved copper (CuCl2), as well as a more rapid copper release compared with CuO NPs. Mitochondrial damage was induced by Cu NPs already after 2 h exposure. Cu NPs induced oxidation at high levels in an acellular ROS assay, and a small increase of intracellular ROS was observed. The increase of DNA damage was limited. CuO NPs did not induce any mitochondrial damage up to 6 h of exposure. No acellular ROS was induced by the CuO NPs, and the levels of intracellular ROS and DNA damage were limited after 2 h exposure. Necrosis was the main type of cell death observed after 18 h exposure to CuO NP and dissolved copper.

Place, publisher, year, edition, pages
Elsevier, 2015. Vol. 29, no 7, 1711-1719 p.
Keyword [en]
Nanoparticles, Cu, CuO, Oxidative stress, Cytotoxicity, Mitochondrial damage
National Category
Pharmacology and Toxicology
Identifiers
URN: urn:nbn:se:kth:diva-174918DOI: 10.1016/j.tiv.2015.05.020ISI: 000361263500044PubMedID: 26028147Scopus ID: 2-s2.0-84938537299OAI: oai:DiVA.org:kth-174918DiVA: diva2:865412
Funder
Swedish Research Council
Note

QC 20151028

Available from: 2015-10-28 Created: 2015-10-09 Last updated: 2017-12-01Bibliographically approved
In thesis
1. Surface reactivity of metal nanoparticles: - importance of surface active agents and biomolecules from a transformation, mobility and toxicity perspective
Open this publication in new window or tab >>Surface reactivity of metal nanoparticles: - importance of surface active agents and biomolecules from a transformation, mobility and toxicity perspective
2017 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Metallic nanoparticles possess unique properties due to their size and are widely used in e.g. consumer products. From this follows a need to identify and assess potential risks of human and environmental exposure. Their size facilitates uptake in organisms and disruption of various biological processes. Together with a high reactivity, mainly due to their large surface area in solution, they are both commonly used in different applications and of a potential safety concern. Risk assessment requires hence in-depth knowledge on the particle characteristics and their behavior in solution but also how these properties change with time and exposure conditions and whether these characteristics can be linked to toxicity following nanoparticle exposure. This thesis addresses these aspects with a main focus on metal nanoparticles and elaborates on the importance of characterization, how such measurements can be done, and on interactions with surfactants and biomolecules and toxic effects.Silver nanoparticles are, due to their antibacterial properties, often used in sportswear to prevent sweat odor. During laundry they may be dispersed and interact with surfactants of the washing powder, influencing their properties and stability in solution. These aspects are addressed in Papers I, III and V on silver nanoparticles of different size and surface coatings. The stability was shown to depend on the surface charge and the concentration of the surfactant. The stability and extent of silver release were reduced upon sequential exposure, indicating the importance of the particle history on their bioaccessibility, mobility and potential toxicity. A mechanism was proposed for how silver nanoparticles are stabilized in surfactant solutions.Toxic effects of silver nanoparticles of different size and coatings on cultivated lung cells, Paper II, and effects of copper-containing nanoparticles on different blood cells, Paper IV, were studied in vitro. The smallest particles were most cytotoxic and the “Trojan horse” mechanism played an important role, meaning that the nanoparticles facilitate cellular uptake followed by ion-release.Difficulties in the determination and interpretation of the zeta potential, related to the surface charge, of metal nanoparticles in complex solutions are elucidated in Paper VI. Guidelines are provided on how to accurately assess this property.

Place, publisher, year, edition, pages
Stockholm: KTH Royal Institute of Technology, 2017. 62 p.
Series
TRITA-CHE-Report, ISSN 1654-1081 ; 2017:16
Keyword
metal nanoparticles, surfactants, metal release, characterization, size distribution, nanotoxicology, risk assessment
National Category
Chemical Sciences
Research subject
Chemistry
Identifiers
urn:nbn:se:kth:diva-199596 (URN)978-91-7729-247-0 (ISBN)
Public defence
2017-02-02, F3, Lindstedtsvägen 26, Stockholm, 10:00 (English)
Opponent
Supervisors
Funder
Swedish Research CouncilSwedish Research Council Formas
Note

QC 20170111

Available from: 2017-01-11 Created: 2017-01-10 Last updated: 2017-03-07Bibliographically approved

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Wallinder, Inger Odnevall

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