Proteomics Analysis Reveals Distinct Corona Composition on Magnetic Nanoparticles with Different Surface Coatings: Implications for Interactions with Primary Human Macrophages
2015 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 10, no 10, e0129008Article in journal (Refereed) Published
Superparamagnetic iron oxide nanoparticles (SPIONs) have emerged as promising contrast agents for magnetic resonance imaging. The influence of different surface coatings on the biocompatibility of SPIONs has been addressed, but the potential impact of the so-called corona of adsorbed proteins on the surface of SPIONs on their biological behavior is less well studied. Here, we determined the composition of the plasma protein corona on silica- coated versus dextran-coated SPIONs using mass spectrometry-based proteomics approaches. Notably, gene ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed distinct protein corona compositions for the two different SPIONs. Relaxivity of silica-coated SPIONs was modulated by the presence of a protein corona. Moreover, the viability of primary human monocyte-derived macrophages was influenced by the protein corona on silica-coated, but not dextran-coated SPIONs, and the protein corona promoted cellular uptake of silica-coated SPIONs, but did not affect internalization of dextran-coated SPIONs.
Place, publisher, year, edition, pages
[Vogt, Carmen; Kohonen, Pekka; Fadeel, Bengt] Karolinska Inst, Inst Environm Med, Div Mol Toxicol, S-10401 Stockholm, Sweden. [Pernemalm, Maria; Lehtio, Janne] Sci Life Lab, Dept Oncol Pathol, Clin Prote Mass Spectrometry, Stockholm, Sweden. [Pernemalm, Maria; Lehtio, Janne] Karolinska Inst, Stockholm, Sweden. [Laurent, Sophie] Univ Mons, Dept Gen Organ & Biomed Chem, NMR & Mol Imaging Lab, B-7000 Mons, Belgium. [Hultenby, Kjell] Karolinska Inst, Dept Lab Med, Electron Microscopy Core Facil, Stockholm, Sweden. [Vahter, Marie] Karolinska Inst, Inst Environm Med, Div Met & Hlth, S-10401 Stockholm, Sweden. [Toprak, Muhammet S.] Royal Inst Technol, Sch Informat & Commun Technol, Funct Mat Div, Stockholm, Sweden., 2015. Vol. 10, no 10, e0129008
Nano Technology Materials Engineering
IdentifiersURN: urn:nbn:se:kth:diva-176341DOI: 10.1371/journal.pone.0129008ISI: 000362510600001PubMedID: 26444829ScopusID: 2-s2.0-84948680390OAI: oai:DiVA.org:kth-176341DiVA: diva2:868013
QC 201511092015-11-092015-11-032015-11-09Bibliographically approved