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Electrochemical microfluidic platform for simultaneous multi-analyte detection
KTH, School of Biotechnology (BIO), Protein Technology.
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2015 (English)In: Eurosensors 2015, Elsevier, 2015, Vol. 120, 916-919 p.Conference paper (Refereed)
Abstract [en]

We present an electrochemical lab-on-a-chip (LOC) platform for the simultaneous detection of up to four different analytes. The possibility to separately immobilize different assays in a channel network, without active valves, was successfully demonstrated using a model assay linked to glucose oxidase. This enables the detection of various analytes even with different assay formats. For the assay immobilization, the channel surface, made out of dry film photoresist (DFR), could be activated by means of EDC/NHS-linker chemistry and used for the covalent binding of primary amines. Cross-sensitivity due to diffusion within the channel network could be experimentally excluded.

Place, publisher, year, edition, pages
Elsevier, 2015. Vol. 120, 916-919 p.
, Procedia Engineering, ISSN 1877-7058 ; 120
Keyword [en]
Immunoassay, microfluidics, electrochemical biosensor, dry-film photoresist, lab-on-a-chip, Multi-analyte, Assays, Bioassay, Glucose oxidase, Glucose sensors, Lab-on-a-chip, Photoresists, Channel surface, Covalent binding, Cross sensitivity, Dry-film photoresists, Electrochemical biosensor, Microfluidic platforms, Multi-analytes, Simultaneous detection, Microfluidics
National Category
Industrial Biotechnology
URN: urn:nbn:se:kth:diva-176106DOI: 10.1016/j.proeng.2015.08.794ISI: 000380499300211ScopusID: 2-s2.0-84941670534OAI: diva2:875940
29th European Conference on Solid-State Transducers, EUROSENSORS 2015, Freiburg, Germany, 6 September 2015 through 9 September 2015., Freiburg, Germany, 6 September 2015 through 9 September 2015

QC 20151202

Available from: 2015-12-02 Created: 2015-11-02 Last updated: 2016-09-20Bibliographically approved

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Horak, Josef
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