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The gut microbiota modulates host amino acid and glutathione metabolism in mice
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab. Chalmers University of Technology, Sweden.
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2015 (English)In: Molecular Systems Biology, ISSN 1744-4292, E-ISSN 1744-4292, Vol. 11, no 10, 834Article in journal (Refereed) Published
Abstract [en]

The gut microbiota has been proposed as an environmental factor that promotes the progression of metabolic diseases. Here, we investigated how the gut microbiota modulates the global metabolic differences in duodenum, jejunum, ileum, colon, liver, and two white adipose tissue depots obtained from conventionally raised (CONV-R) and germ-free (GF) mice using gene expression data and tissue-specific genome-scale metabolic models (GEMs). We created a generic mouse metabolic reaction (MMR) GEM, reconstructed 28 tissue-specific GEMs based on proteomics data, and manually curated GEMs for small intestine, colon, liver, and adipose tissues. We used these functional models to determine the global metabolic differences between CONV-R and GF mice. Based on gene expression data, we found that the gut microbiota affects the host amino acid (AA) metabolism, which leads to modifications in glutathione metabolism. To validate our predictions, we measured the level of AAs and N-acetylated AAs in the hepatic portal vein of CONV-R and GF mice. Finally, we simulated the metabolic differences between the small intestine of the CONV-R and GF mice accounting for the content of the diet and relative gene expression differences. Our analyses revealed that the gut microbiota influences host amino acid and glutathione metabolism in mice.

Place, publisher, year, edition, pages
Blackwell Publishing, 2015. Vol. 11, no 10, 834
Keyword [en]
genome-scale metabolic models, germ-free mice, glutathione metabolism, metabolomics, transcriptomics
National Category
Biochemistry and Molecular Biology
URN: urn:nbn:se:kth:diva-177963DOI: 10.15252/msb.20156487ISI: 000364315500006PubMedID: 26475342ScopusID: 2-s2.0-84945903211OAI: diva2:876348

QC 20151203

Available from: 2015-12-03 Created: 2015-11-30 Last updated: 2015-12-03Bibliographically approved

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Mardinoglu, AdilNielsen, Jens
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