Size controlled protein nanoemulsions for active targeting of folate receptor positive cells
2015 (English)In: Colloids and Surfaces B: Biointerfaces, ISSN 0927-7765, E-ISSN 1873-4367, Vol. 135, 90-98 p.Article in journal (Refereed) Published
Bovine serum albumin (BSA) nanoemulsions were produced by high pressure homogenization with a tri-block copolymer (Poloxamer 407), which presents a central hydrophobic chain of polyoxypropylene (PPO) and two identical lateral hydrophilic chains of polyethylene glycol (PEG). We observed a linear correlation between tri-block copolymer concentration and size - the use of 5. mg/mL of Poloxamer 407 yields nanoemulsions smaller than 100. nm. Molecular dynamics and fluorescent tagging of the tri-block copolymer highlight their mechanistic role on the size of emulsions. This novel method enables the fabrication of highly stable albumin emulsions in the nano-size range, highly desirable for controlled drug delivery. Folic Acid (FA)-tagged protein nanoemulsions were shown to promote specific folate receptor (FR)-mediated targeting in FR positive cells. The novel strategy presented here enables the construction of size controlled, functionalized protein-based nanoemulsions with excellent characteristics for active targeting in cancer therapy.
Place, publisher, year, edition, pages
Elsevier, 2015. Vol. 135, 90-98 p.
IdentifiersURN: urn:nbn:se:kth:diva-174582DOI: 10.1016/j.colsurfb.2015.06.073ISI: 000367410200011ScopusID: 2-s2.0-84938412704OAI: oai:DiVA.org:kth-174582DiVA: diva2:877581
QC 201512072015-12-072015-10-072016-01-26Bibliographically approved