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Semisynthesis of fluorescent metabolite sensors on cell surfaces.
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2011 (English)In: Journal of the American Chemical Society, ISSN 0002-7863, E-ISSN 1520-5126, Vol. 133, no 40Article in journal (Refereed) Published
Abstract [en]

Progress in understanding signal transduction and metabolic pathways is hampered by a shortage of suitable sensors for tracking metabolites, second messengers, and neurotransmitters in living cells. Here we introduce a class of rationally designed semisynthetic fluorescent sensor proteins, called Snifits, for measuring metabolite concentrations on the cell surface of mammalian cells. Functional Snifits are assembled on living cells through two selective chemical labeling reactions of a genetically encoded protein scaffold. Our best Snifit displayed fluorescence intensity ratio changes on living cells significantly higher than any previously reported cell-surface-targeted fluorescent sensor protein. This work establishes a generally applicable and rational strategy for the generation of cell-surface-targeted fluorescent sensor proteins for metabolites of interest.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2011. Vol. 133, no 40
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Biochemistry and Molecular Biology Analytical Chemistry
URN: urn:nbn:se:kth:diva-178095DOI: 10.1021/ja206915mISI: 000296036700069PubMedID: 21879732ScopusID: 2-s2.0-80053525802OAI: diva2:877624

QC 20151215

Available from: 2015-12-07 Created: 2015-12-07 Last updated: 2015-12-15Bibliographically approved

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Piguet, Joachim
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