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Expression Profiles of Estrogen-Regulated MicroRNAs in Breast Cancer Cells
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. Department of Biology and Biochemistry, University of Houston, Houston.ORCID iD: 0000-0002-0602-2062
2016 (English)In: Methods in Molecular Biology: The Estrogen Receptors / [ed] Kathleen M. Eyster, Springer, 2016, Vol. 1366Chapter in book (Other academic)
Abstract [en]

Molecular signaling through both estrogen and microRNAs are critical for breast cancer development and growth. The activity of estrogen is mediated by transcription factors, the estrogen receptors. Here we describe a method for robust characterization of estrogen-regulated microRNA profiles. The method details how to prepare cells for optimal estrogen response, directions for estrogen treatment, RNA extraction, microRNA large-scale profiling, and subsequent confirmations.

Place, publisher, year, edition, pages
Springer, 2016. Vol. 1366
Keyword [en]
Receptor Tyrosine Kinase, Mammary Epithelial-Cells, Activated Protein-Kinase, Mesenchymal Transition, Posttranscriptional Regulation, Gene-Expression, Stem-Cells, Microrna, Progression, Family
National Category
Natural Sciences Biological Sciences Cell and Molecular Biology
URN: urn:nbn:se:kth:diva-178111DOI: 10.1007/978-1-4939-3127-9_30PubMedID: 26585151ScopusID: 2-s2.0-84947781743OAI: diva2:877769

QC 20151214

Available from: 2015-12-07 Created: 2015-12-07 Last updated: 2015-12-14Bibliographically approved

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