Micro-droplet based directed evolution outperforms conventional laboratory evolution
2014 (English)In: 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, Chemical and Biological Microsystems Society , 2014, 169-171 p.Conference paper (Refereed)
We present droplet adaptive laboratory evolution (DrALE), a directed evolution method used to improve industrial enzyme producing microorganisms for e.g. feedstock digestion. DrALE is based linking a desired phenotype to growth rate allowing only desired cells to proliferate. Single cells are confined in microfluidic droplets to prevent the phenotype, e.g. secreted enzymes, from leaking between cells. The method was benchmarked against and found to significantly outperform conventional adaptive laboratory evolution (ALE) in enriching enzyme producing cells. It was furthermore applied to enrich a whole-genome mutated library of yeast cells for α-amylase activity.
Place, publisher, year, edition, pages
Chemical and Biological Microsystems Society , 2014. 169-171 p.
Cell factories, Directed evolution, Droplet microfluidics, Enzymes, High throughput, Cytology, Drops, Microfluidics, Alpha-amylase activity, Directed evolution methods, Industrial enzymes, Micro droplets, Microfluidic droplets, Cells
Immunology Cell and Molecular Biology
IdentifiersURN: urn:nbn:se:kth:diva-174791ScopusID: 2-s2.0-84941686278ISBN: 9780979806476OAI: oai:DiVA.org:kth-174791DiVA: diva2:878266
18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, 26 October 2014 through 30 October 2014
QC 201512082015-12-082015-10-072015-12-08Bibliographically approved