In situ isothermal rolling circle amplification (RCA) of DNA and bead based visualization of RCA products on an integrated lab on DVD platform for low cost molecular diagnostics
2014 (English)In: 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, 2014, 1422-1424 p.Conference paper (Refereed)
Isothermal rolling circle amplification (RCA) is a simple and versatile isothermal enzymatic nucleic acid amplification techniques, and has been demonstrated as a ideal tool for biomedical research. We demonstrate for the first time the detection of padlock probes mediated and rolling circle amplification of DNA on centrifugal microfluidic system. DNA spotted on DVD substrate is amplified in-situ and novel bead-based detection of the amplified product is demonstrated using a modified commercial DVD reader for "image based" molecular diagnostics.
Place, publisher, year, edition, pages
2014. 1422-1424 p.
Centrifugal microfluidics, Molecular diagnostics, Rolling circle amplification, Centrifugation, Data storage equipment, DNA, Isotherms, Nucleic acids, Videodisks, Amplification technique, Bead-based, Biomedical research, Image-based, Isothermal rolling, Rolling circle amplifications, Microfluidics
Other Industrial Biotechnology
IdentifiersURN: urn:nbn:se:kth:diva-174797ScopusID: 2-s2.0-84941687313ISBN: 9780979806476OAI: oai:DiVA.org:kth-174797DiVA: diva2:878320
18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, 26 October 2014 through 30 October 2014
QC 201512082015-12-082015-10-072015-12-08Bibliographically approved