Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
In situ isothermal rolling circle amplification (RCA) of DNA and bead based visualization of RCA products on an integrated lab on DVD platform for low cost molecular diagnostics
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology.ORCID iD: 0000-0001-5199-0663
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology.
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology.
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology.
2014 (English)In: 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, 2014, 1422-1424 p.Conference paper, Published paper (Refereed)
Abstract [en]

Isothermal rolling circle amplification (RCA) is a simple and versatile isothermal enzymatic nucleic acid amplification techniques, and has been demonstrated as a ideal tool for biomedical research. We demonstrate for the first time the detection of padlock probes mediated and rolling circle amplification of DNA on centrifugal microfluidic system. DNA spotted on DVD substrate is amplified in-situ and novel bead-based detection of the amplified product is demonstrated using a modified commercial DVD reader for "image based" molecular diagnostics.

Place, publisher, year, edition, pages
2014. 1422-1424 p.
Keyword [en]
Centrifugal microfluidics, Molecular diagnostics, Rolling circle amplification, Centrifugation, Data storage equipment, DNA, Isotherms, Nucleic acids, Videodisks, Amplification technique, Bead-based, Biomedical research, Image-based, Isothermal rolling, Rolling circle amplifications, Microfluidics
National Category
Other Industrial Biotechnology
Identifiers
URN: urn:nbn:se:kth:diva-174797Scopus ID: 2-s2.0-84941687313ISBN: 9780979806476 (print)OAI: oai:DiVA.org:kth-174797DiVA: diva2:878320
Conference
18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, 26 October 2014 through 30 October 2014
Note

QC 20151208

Available from: 2015-12-08 Created: 2015-10-07 Last updated: 2015-12-08Bibliographically approved

Open Access in DiVA

No full text

Scopus

Search in DiVA

By author/editor
Ramachandraiah, HarishaZelenin, SergeyAmasia, MaryRussom, Aman
By organisation
Proteomics and Nanobiotechnology
Other Industrial Biotechnology

Search outside of DiVA

GoogleGoogle Scholar

isbn
urn-nbn

Altmetric score

isbn
urn-nbn
Total: 92 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf