Real-time monitoring of melting curves on DNA microarrays in plastic lab-on-foil system using silicon photomultiplier detectors
2014 (English)In: 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, Chemical and Biological Microsystems Society , 2014, 2294-2296 p.Conference paper (Refereed)
We report on DNA genotyping by melting curve analysis (MCA) in a plastic foil-microfluidic system using silicon photomultiplier (SiPM) detectors. Matching and single base mismatching DNA oligonucleotides were immobilized on thin-film foil microheaters and integrated in microfluidic channels using lamination foils. Two SiPMs, each with a sensitive area of 1.2×1.2 mm were assembled in an optical measurement setup to monitor the decaying Cy3 fluorescence intensity of the hybridized DNA spots upon melting. Successful detection of single nucleotide polymorphisms (SNP) is demonstrated.
Place, publisher, year, edition, pages
Chemical and Biological Microsystems Society , 2014. 2294-2296 p.
Lab-on-chip, Real-time melting curve analysis, Silicon photomultiplier, SNP detection, Bioassay, Biochips, DNA, Fluidic devices, Melting, Microfluidics, Oligonucleotides, Optical data processing, Photomultipliers, Silicon, Silicon detectors, DNA oligonucleotides, Fluorescence intensities, Lab on chip, Melting curve analysis, Micro fluidic system, Microfluidic channel, Single-nucleotide polymorphisms, Microarrays
IdentifiersURN: urn:nbn:se:kth:diva-174803ScopusID: 2-s2.0-84941711431ISBN: 9780979806476OAI: oai:DiVA.org:kth-174803DiVA: diva2:881331
18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, 26 October 2014 through 30 October 2014
QC 201512102015-12-102015-10-072015-12-10Bibliographically approved