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Solid tumor spheroid formation by temperature-controlled high voltage ultrasound in a multi-well microdevice
KTH, School of Engineering Sciences (SCI), Applied Physics, Cell Physics.
KTH, School of Engineering Sciences (SCI), Applied Physics.ORCID iD: 0000-0002-7023-4772
KTH, School of Engineering Sciences (SCI), Applied Physics, Cell Physics.ORCID iD: 0000-0001-5178-7593
KTH, School of Engineering Sciences (SCI), Applied Physics, Biomedical and X-ray Physics.
2014 (English)In: 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, Chemical and Biological Microsystems SocietyChemical and Biological Microsystems Society , 2014, 573-575 p.Conference paper (Refereed)
Abstract [en]

In the present work we demonstrate effective 3D growth of human hepatocellular carcinoma (HCC) HepG2 cell spheroids in parallel in a multi-well microdevice actuated with high voltage ultrasound in a temperature-controlled system. We compare the spheroid formation during continuous ultrasound exposure for one week where we formed spheroids in 59% of the wells, with the spheroid formation without ultrasound actuation, where we obtained 0% spheroids. Furthermore, we present an application of the tumor spheroids for investigating natural killer (NK) cells behavior against solid tumors.

Place, publisher, year, edition, pages
Chemical and Biological Microsystems SocietyChemical and Biological Microsystems Society , 2014. 573-575 p.
Keyword [en]
Microchip, Multi-well, Natural killer cells, Solid tumor, Spheroid, Ultrasound, Temperature control, Tumors, Multi wells, Solid tumors, Ultrasonics
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:kth:diva-174813ScopusID: 2-s2.0-84941633758ISBN: 9780979806476OAI: oai:DiVA.org:kth-174813DiVA: diva2:881498
Conference
18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, 26 October 2014 through 30 October 2014
Note

QC 20151210

Available from: 2015-12-10 Created: 2015-10-07 Last updated: 2015-12-10Bibliographically approved

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Christakou, Athanasia E.Ohlin, MathiasÖnfelt, BjörnWiklund, Martin
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