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High density custom microarrays formed by microcompartment amplification on glass surface
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology.
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2014 (English)In: 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, Chemical and Biological Microsystems Society , 2014, 1027-1029 p.Conference paper, Published paper (Refereed)
Abstract [en]

Compartmentalization of a single DNA molecule is necessary for digital amplification. In the present study we have developed a microscale isothermal amplification using exponential rolling circle amplification (RCA). RCA was performed in PDMS microcompartments on a microarray glass, with a volume of less than 1 pL. Resulting amplicons were attached to the glass surface and formed a custom array with the density of spots above 2,5 × 105 per cm2. Our technology can be applied for digital amplification of DNA or RNA from a variety of complex biological samples in a microchip format.

Place, publisher, year, edition, pages
Chemical and Biological Microsystems Society , 2014. 1027-1029 p.
Keyword [en]
Digital, Microcompartments, RCA, Solid surface amplification, Bioassay, Microarrays, Nucleic acids, Biological samples, Isothermal amplifications, Micro-compartments, Rolling circle amplifications, Single DNA molecules, Solid surface, Glass
National Category
Physical Chemistry Other Basic Medicine Analytical Chemistry
Identifiers
URN: urn:nbn:se:kth:diva-174808Scopus ID: 2-s2.0-84941662700ISBN: 9780979806476 (print)OAI: oai:DiVA.org:kth-174808DiVA: diva2:881628
Conference
18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, 26 October 2014 through 30 October 2014
Note

QC 20151211

Available from: 2015-12-11 Created: 2015-10-07 Last updated: 2015-12-11Bibliographically approved

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