High density custom microarrays formed by microcompartment amplification on glass surface
2014 (English)In: 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, Chemical and Biological Microsystems Society , 2014, 1027-1029 p.Conference paper (Refereed)
Compartmentalization of a single DNA molecule is necessary for digital amplification. In the present study we have developed a microscale isothermal amplification using exponential rolling circle amplification (RCA). RCA was performed in PDMS microcompartments on a microarray glass, with a volume of less than 1 pL. Resulting amplicons were attached to the glass surface and formed a custom array with the density of spots above 2,5 × 105 per cm2. Our technology can be applied for digital amplification of DNA or RNA from a variety of complex biological samples in a microchip format.
Place, publisher, year, edition, pages
Chemical and Biological Microsystems Society , 2014. 1027-1029 p.
Digital, Microcompartments, RCA, Solid surface amplification, Bioassay, Microarrays, Nucleic acids, Biological samples, Isothermal amplifications, Micro-compartments, Rolling circle amplifications, Single DNA molecules, Solid surface, Glass
Physical Chemistry Other Basic Medicine Analytical Chemistry
IdentifiersURN: urn:nbn:se:kth:diva-174808ScopusID: 2-s2.0-84941662700ISBN: 9780979806476OAI: oai:DiVA.org:kth-174808DiVA: diva2:881628
18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, 26 October 2014 through 30 October 2014
QC 201512112015-12-112015-10-072015-12-11Bibliographically approved