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Host-derived pericytes and Sca-1+ cells predominate in the MART-1 stroma fraction of experimentally induced melanoma
Dept of Environmental Health, Harvard School of Public Health.
Dept of Environmental Health, Harvard School of Public Health.
Dept of Environmental Health, Harvard School of Public Health.
Dept of Environmental Health, Harvard School of Public Health.
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2011 (English)In: Journal of Histochemistry and Cytochemistry, ISSN 0022-1554, E-ISSN 1551-5044, Vol. 59, 1060-1075 p.Article in journal (Refereed) Published
Abstract [en]

Identification of cell types in tumor-associated stroma that are involved in the development of melanoma is hampered by their heterogeneity. The authors used flow cytometry and immunohistochemistry to demonstrate that anti-MART-1 antibodies can discriminate between melanoma and stroma cells. They investigated the cellular composition of the MART-1-, non-hematopoietic melanoma-associated stroma, finding it consisted mainly of Sca-1+ and CD146+ cells. These cell types were also observed in the skin and muscle adjacent to developing melanomas. The Sca-1+ cell population was observed distributed in the epidermis, hair follicle bulges, and tumor capsule. The CD146+ population was found distributed within the tumor, mainly associated with blood vessels in a perivascular location. In addition to a perivascular distribution, CD146+ cells expressed α-smooth muscle actin, lacked expression of endothelial markers CD31 and CD34, and were therefore identified as pericytes. Pericytes were found to be associated with CD31+ endothelial cells; however, some pericytes were also observed associated with CD31-, MART-1+ B16 melanoma cells that appeared to form blood vessel structures. Furthermore, the authors observed extensive nuclear expression of HIF-1α in melanoma and stroma cells, suggesting hypoxia is an important factor associated with the melanoma microenvironment and vascularization. The results suggest that pericytes and Sca-1+ stroma cells are important contributors to melanoma development.

Place, publisher, year, edition, pages
2011. Vol. 59, 1060-1075 p.
Keyword [en]
MART-1, Sca-1, CD146, pericytes, melanoma, tumor-associated stroma
National Category
Cell and Molecular Biology Cancer and Oncology
URN: urn:nbn:se:kth:diva-179191DOI: 10.1369/0022155411428078ISI: 000297649800002PubMedID: 3283083ScopusID: 2-s2.0-84856012298OAI: diva2:881668

QC 20160226

Available from: 2015-12-11 Created: 2015-12-11 Last updated: 2016-02-26Bibliographically approved

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