Host-derived pericytes and Sca-1+ cells predominate in the MART-1− stroma fraction of experimentally induced melanoma
2011 (English)In: Journal of Histochemistry and Cytochemistry, ISSN 0022-1554, E-ISSN 1551-5044, Vol. 59, 1060-1075 p.Article in journal (Refereed) Published
Identification of cell types in tumor-associated stroma that are involved in the development of melanoma is hampered by their heterogeneity. The authors used flow cytometry and immunohistochemistry to demonstrate that anti-MART-1 antibodies can discriminate between melanoma and stroma cells. They investigated the cellular composition of the MART-1-, non-hematopoietic melanoma-associated stroma, finding it consisted mainly of Sca-1+ and CD146+ cells. These cell types were also observed in the skin and muscle adjacent to developing melanomas. The Sca-1+ cell population was observed distributed in the epidermis, hair follicle bulges, and tumor capsule. The CD146+ population was found distributed within the tumor, mainly associated with blood vessels in a perivascular location. In addition to a perivascular distribution, CD146+ cells expressed α-smooth muscle actin, lacked expression of endothelial markers CD31 and CD34, and were therefore identified as pericytes. Pericytes were found to be associated with CD31+ endothelial cells; however, some pericytes were also observed associated with CD31-, MART-1+ B16 melanoma cells that appeared to form blood vessel structures. Furthermore, the authors observed extensive nuclear expression of HIF-1α in melanoma and stroma cells, suggesting hypoxia is an important factor associated with the melanoma microenvironment and vascularization. The results suggest that pericytes and Sca-1+ stroma cells are important contributors to melanoma development.
Place, publisher, year, edition, pages
2011. Vol. 59, 1060-1075 p.
MART-1, Sca-1, CD146, pericytes, melanoma, tumor-associated stroma
Cell and Molecular Biology Cancer and Oncology
IdentifiersURN: urn:nbn:se:kth:diva-179191DOI: 10.1369/0022155411428078ISI: 000297649800002PubMedID: 3283083ScopusID: 2-s2.0-84856012298OAI: oai:DiVA.org:kth-179191DiVA: diva2:881668
QC 201602262015-12-112015-12-112016-02-26Bibliographically approved