Controlled Lateral Positioning of Microparticles Inside Droplets Using Acoustophoresis
2015 (English)In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 87, no 20, 10521-10526 p.Article in journal (Refereed) Published
In this paper, we utilize bulk acoustic waves to control the position of micropartides inside droplets in two-phase microfluidic systems and demonstrate a method to enrich the micropartides. In droplet microfluidics, different unit operations are combined and integrated on-chip to miniaturize complex biochemical assays. We present a droplet unit operation capable of controlling the position of micropartides during a trident shaped droplet split. An acoustic standing wave field is generated in the microchannel, and the acoustic forces direct the encapsulated micropartides to the center of the droplets. The method is generic, requires no labeling of the micropartides, and is operated in a noncontact fashion. It was possible to achieve 2+-fold enrichment of polystyrene beads (5 mu m in diameter) in the center daughter droplet with an average recovery of 89% of the beads. Red blood cells were also successfully manipulated inside droplets. These results show the possibility to use acoustophoresis in two-phase systems to enrich micropartides and open up the possibility for new droplet-based assays that are not performed today.
Place, publisher, year, edition, pages
American Chemical Society (ACS), 2015. Vol. 87, no 20, 10521-10526 p.
Bioassay, Biochips, Microarrays, Microfluidics, Position control, Acoustic standing wave, Biochemical assay, Bulk acoustic waves, Daughter droplets, Droplet microfluidics, Micro fluidic system, Polystyrene beads, Two phase systems
Robotics Other Electrical Engineering, Electronic Engineering, Information Engineering
IdentifiersURN: urn:nbn:se:kth:diva-176960DOI: 10.1021/acs.analchem.5b02746ISI: 000363348300051PubMedID: 26422760ScopusID: 2-s2.0-84945288371OAI: oai:DiVA.org:kth-176960DiVA: diva2:883065
QC 201512162015-12-162015-11-132016-09-19Bibliographically approved