Single-cell screening of photosynthetic growth and lactate production by cyanobacteria
2015 (English)In: Biotechnology for Biofuels, ISSN 1754-6834, E-ISSN 1754-6834, Vol. 8, 193Article in journal (Refereed) PublishedText
Background: Photosynthetic cyanobacteria are attractive for a range of biotechnological applications including biofuel production. However, due to slow growth, screening of mutant libraries using microtiter plates is not feasible. Results: We present a method for high-throughput, single-cell analysis and sorting of genetically engineered l-lactate-producing strains of Synechocystis sp. PCC6803. A microfluidic device is used to encapsulate single cells in picoliter droplets, assay the droplets for L-lactate production, and sort strains with high productivity. We demonstrate the separation of low- and high-producing reference strains, as well as enrichment of a more productive L-lactate-synthesizing population after UV-induced mutagenesis. The droplet platform also revealed population heterogeneity in photosynthetic growth and lactate production, as well as the presence of metabolically stalled cells. Conclusions: The workflow will facilitate metabolic engineering and directed evolution studies and will be useful in studies of cyanobacteria biochemistry and physiology.
Place, publisher, year, edition, pages
BioMed Central, 2015. Vol. 8, 193
Cyanobacteria, Single cell, Droplet microfluidics, High-throughput screening, Lactate dehydrogenase
IdentifiersURN: urn:nbn:se:kth:diva-180126DOI: 10.1186/s13068-015-0380-2ISI: 000365784000009ScopusID: 2-s2.0-84947929588OAI: oai:DiVA.org:kth-180126DiVA: diva2:894074
QC 201601142016-01-142016-01-072016-01-14Bibliographically approved