Optical depletion mechanism of upconverting luminescence and its potential for multi-photon STED-like microscopy
2015 (English)In: Optics Express, ISSN 1094-4087, E-ISSN 1094-4087, Vol. 23, no 25, 32401-32412 p.Article in journal (Refereed) PublishedText
Simulated emission depletion (STED) microscopy is very powerful, but still suffers from small tissue penetration depth, photobleaching of fluorescent probes and complicated imaging systems. Here, we propose an optical luminescence depletion mechanism employing upconverting nanoparticles (UCNPs) and explore its potential for multi-photon STED-like microscopy. With the addition of Yb3+ ions in NaYF4:Er3+ UCNPs, the two-photon green emission of Er3+ under 795-nm excitation was successfully depleted by 1140-nm laser through the synergetic effect of the excited state absorption and the interionic energy transfer. This STED-like depletion mechanism was systematically investigated using steady-state rate equations, evidenced by the surprising emerging of 478-nm emission. The green emission depletion efficiency was about 30%, limited by the current laser source. Our work indicates that NaYF4:Yb3+/Er3+ UCNPs will be potential probes for multi-photon super-resolution microscopy with many advantages, including long-wavelength-induced large penetration, non-photobleaching and non-photoblinking properties, cost-effective and simplified imaging systems.
Place, publisher, year, edition, pages
Optical Society of America, 2015. Vol. 23, no 25, 32401-32412 p.
Atom and Molecular Physics and Optics
IdentifiersURN: urn:nbn:se:kth:diva-180497DOI: 10.1364/OE.23.032401ISI: 000366687200075PubMedID: 26699029ScopusID: 2-s2.0-84959420286OAI: oai:DiVA.org:kth-180497DiVA: diva2:895626
QC 201601192016-01-192016-01-142016-01-19Bibliographically approved