Change search
ReferencesLink to record
Permanent link

Direct link
Versatile microscale screening platform for improving recombinant protein productivity in Chinese hamster ovary cells
Show others and affiliations
2015 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 5, 18016Article in journal (Refereed) PublishedText
Abstract [en]

Chinese hamster ovary (CHO) cells are widely used as cell factories for the production of biopharmaceuticals. In contrast to the highly optimized production processes for monoclonal antibody (mAb)-based biopharmaceuticals, improving productivity of non-mAb therapeutic glycoproteins is more likely to reduce production costs significantly. The aim of this study was to establish a versatile target gene screening platform for improving productivity for primarily non-mAb glycoproteins with complete interchangeability of model proteins and target genes using transient expression. The platform consists of four techniques compatible with 96-well microplates: lipid-based transient transfection, cell cultivation in microplates, cell counting and antibody-independent product titer determination based on split-GFP complementation. We were able to demonstrate growth profiles and volumetric productivity of CHO cells in 96-half-deepwell microplates comparable with those obtained in shake flasks. In addition, we demonstrate that split-GFP complementation can be used to accurately measure relative titers of therapeutic glycoproteins. Using this platform, we were able to detect target gene-specific increase in titer and specific productivity of two non-mAb glycoproteins. In conclusion, the platform provides a novel miniaturized and parallelisable solution for screening target genes and holds the potential to unravel genes that can enhance the secretory capacity of CHO cells.

Place, publisher, year, edition, pages
Nature Publishing Group, 2015. Vol. 5, 18016
National Category
Industrial Biotechnology
URN: urn:nbn:se:kth:diva-180214DOI: 10.1038/srep18016ISI: 000366190500001PubMedID: 26657798ScopusID: 2-s2.0-84949656277OAI: diva2:895659

QC 20160119

Available from: 2016-01-19 Created: 2016-01-08 Last updated: 2016-01-19Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMedScopus

Search in DiVA

By author/editor
Lundqvist, MagnusRockberg, Johan
By organisation
Proteomics and Nanobiotechnology
In the same journal
Scientific Reports
Industrial Biotechnology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 44 hits
ReferencesLink to record
Permanent link

Direct link