Exploring water as building bricks in enzyme engineering
2015 (English)In: Chemical Communications, ISSN 1359-7345, E-ISSN 1364-548X, Vol. 51, no 97, 17221-17224 p.Article in journal (Refereed) PublishedText
A novel enzyme engineering strategy for accelerated catalysis based on redesigning a water network through protein backbone deshielding is presented. Fundamental insight into the energetic consequences associated with the design is discussed in the light of experimental results and computer simulations. Using water as biobricks provides unique opportunities when transition state stabilisation is not easily attained by traditional enzyme engineering.
Place, publisher, year, edition, pages
Royal Society of Chemistry, 2015. Vol. 51, no 97, 17221-17224 p.
IdentifiersURN: urn:nbn:se:kth:diva-180600DOI: 10.1039/c5cc07162cISI: 000366954800004PubMedID: 26426706ScopusID: 2-s2.0-84948408732OAI: oai:DiVA.org:kth-180600DiVA: diva2:896177
FunderSwedish Research Council, 621-2013-5138
QC 201601202016-01-202016-01-192016-02-11Bibliographically approved