Enhancing biological nitrogen removal from tannery effluent by using the efficient Brachymonas denitrificans in pilot plant operations
2005 (English)In: World Journal of Microbiology & Biotechnology, ISSN 0959-3993, E-ISSN 1573-0972, Vol. 21, no 4, 545-552 p.Article in journal (Refereed) Published
Laboratory scale and pilot plant reactors were inoculated with an efficient denitrifier, Brachymonas denitrificans (CCUG 45880), in order to evaluate whether a bio-augmentation approach can be used to enhance biological nitrogen removal from tannery effluents. To determine the effectiveness of the introduced strain, denitrifying activity in the activated sludge was monitored by nitrate uptake rate ( NUR) measurement of NO3-N. Fluorescent in situ hybridization ( FISH) technique was used to monitor the growth of the augmented species. The laboratory scale nitrate removal efficiency with the introduced B. denitrificans (3.7 +/- 0.6 mg NO3-N gVSS(-1) h(-1)) was higher than that of the activated sludge without the addition of the bacteria (3.5 +/- 0.7 mg NO3-N gVSS(-1) h(-1)); the NUR in the pilot plant after and before the introduction of the strain was also of the magnitude of 12.0 +/- 1.4 and 10.6 +/- 1.4 mg NO3- N gVSS(-1) day(-1), respectively. In situ hybridization results revealed that the introduced denitrifying bacteria significantly facilitated the development of a dense denitrifying bacterial population in the activated sludge, which enhanced in situ denitrification activity. FISH data indicated that once introduced, B. denitrificans remained abundant throughout the experimental period. The ability to seed a bioreactor with bacterial strain capable of removing target pollutants from tannery effluents in a mixed microbial community suggests that this approach could have commercial applications.
Place, publisher, year, edition, pages
2005. Vol. 21, no 4, 545-552 p.
bio-augmentation, denitrifying bacteria, fluorescent in situ hybridization, nitrate uptake rate
IdentifiersURN: urn:nbn:se:kth:diva-5341DOI: 10.1007/s11274-004-3272-3ISI: 000230731500027ScopusID: 2-s2.0-22944446737OAI: oai:DiVA.org:kth-5341DiVA: diva2:8973