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Annular dark field transmission electron microscopy for protein structure determination
KTH, School of Technology and Health (STH), Basic Science and Biomedicine, Structural Biotechnology. Department of Biosciences and Nutrition, Novum, Huddinge, Sweden.
2016 (English)In: Ultramicroscopy, ISSN 0304-3991, E-ISSN 1879-2723, Vol. 161, 98-104 p.Article in journal (Refereed) PublishedText
Abstract [en]

Recently annular dark field (ADF) transmission electron microscopy (TEM) has been advocated as a means of recording images of biological specimens with better signal to noise ratio (SNR) than regular bright field images. I investigate whether and how such images could be used to determine the three-dimensional structure of proteins given that an ADF aperture with a suitable pass-band can be manufactured and used in practice. I develop an approximate theory of ADF-TEM image formation for weak amplitude and phase objects and test this theory using computer simulations. I also test whether these simulated images can be used to calculate a three-dimensional model of the protein using standard software and discuss problems and possible ways to overcome these.

Place, publisher, year, edition, pages
Elsevier, 2016. Vol. 161, 98-104 p.
Keyword [en]
Annular dark field aperture, Linear imaging, Phase contrast transfer function, Projection approximation, Resolution, Transmission electron microscopy
National Category
Medical Image Processing
Identifiers
URN: urn:nbn:se:kth:diva-180925DOI: 10.1016/j.ultramic.2015.11.010ISI: 000367357500013ScopusID: 2-s2.0-84949266631OAI: oai:DiVA.org:kth-180925DiVA: diva2:897839
Note

QC 20160126

Available from: 2016-01-26 Created: 2016-01-25 Last updated: 2016-01-26Bibliographically approved

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