Annular dark field transmission electron microscopy for protein structure determination
2016 (English)In: Ultramicroscopy, ISSN 0304-3991, E-ISSN 1879-2723, Vol. 161, 98-104 p.Article in journal (Refereed) PublishedText
Recently annular dark field (ADF) transmission electron microscopy (TEM) has been advocated as a means of recording images of biological specimens with better signal to noise ratio (SNR) than regular bright field images. I investigate whether and how such images could be used to determine the three-dimensional structure of proteins given that an ADF aperture with a suitable pass-band can be manufactured and used in practice. I develop an approximate theory of ADF-TEM image formation for weak amplitude and phase objects and test this theory using computer simulations. I also test whether these simulated images can be used to calculate a three-dimensional model of the protein using standard software and discuss problems and possible ways to overcome these.
Place, publisher, year, edition, pages
Elsevier, 2016. Vol. 161, 98-104 p.
Annular dark field aperture, Linear imaging, Phase contrast transfer function, Projection approximation, Resolution, Transmission electron microscopy
Medical Image Processing
IdentifiersURN: urn:nbn:se:kth:diva-180925DOI: 10.1016/j.ultramic.2015.11.010ISI: 000367357500013ScopusID: 2-s2.0-84949266631OAI: oai:DiVA.org:kth-180925DiVA: diva2:897839
QC 201601262016-01-262016-01-252016-01-26Bibliographically approved