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Ultrarapid synthesis of microbeads with tuneable surface probe density
KTH, School of Electrical Engineering (EES), Micro and Nanosystems. (Microfluidics)ORCID iD: 0000-0002-9418-452X
KTH, School of Electrical Engineering (EES), Micro and Nanosystems. (Microfluidicis)
KTH, School of Electrical Engineering (EES), Micro and Nanosystems. (Microfluidics)
KTH, School of Electrical Engineering (EES), Micro and Nanosystems. (Micro and Nanosystems)ORCID iD: 0000-0001-8248-6670
Show others and affiliations
2016 (English)In: Ultrarapid synthesis of microbeads with tuneable surface probe density, Shanghai, China: IEEE conference proceedings, 2016, Vol. 2016, 731-734 p.Conference paper, Published paper (Refereed)
Abstract [en]

We present, for the first time, a simplified and ultrarapid (~ 1s) approach for synthesis of microbeads with thiol- functionalized surfaces as potential binding sites, whose density can be tuned according to the ratio of two components in the prepolymer. We verify the successful generation of microbeads and their tuneable surface density resulted from different prepolymer off-stoichiometric ratio. This novel approach can be used for bioassays that require rapid surface capture probe binding and customized probe density for suitable bioreactions.

Place, publisher, year, edition, pages
Shanghai, China: IEEE conference proceedings, 2016. Vol. 2016, 731-734 p.
Series
Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), ISSN 1084-6999 ; 2016
Keyword [en]
microbeads, microfluidics, off-stoichiometry thiol-ene, OSTE
National Category
Medical and Health Sciences
Research subject
Materials Science and Engineering
Identifiers
URN: urn:nbn:se:kth:diva-181779DOI: 10.1109/MEMSYS.2016.7421732ISI: 000381797300192Scopus ID: 2-s2.0-84970996576ISBN: 978-150901973-1 (print)OAI: oai:DiVA.org:kth-181779DiVA: diva2:900116
Conference
29th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2016, Shanghai, China, 24 January 2016 through 28 January 2016
Projects
Theracage
Funder
Stockholm County Council, 66673Swedish Childhood Cancer Foundation, 66673
Note

QC 20161018

Available from: 2016-02-03 Created: 2016-02-03 Last updated: 2017-11-22Bibliographically approved
In thesis
1. OSTE Microfluidic Technologies for Cell Encapsulation and Biomolecular Analysis
Open this publication in new window or tab >>OSTE Microfluidic Technologies for Cell Encapsulation and Biomolecular Analysis
2017 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

In novel drug delivery system, the encapsulation of therapeutic cells in microparticles has great promises for the treatment of a range of health con- ditions. Therefore, the encapsulation material and technology are of great importance to the validity and efficiency of the advanced medical therapy. Several unsolved challenges in regards to versatile microparticle synthesis ma- terials and methods form the main obstacle for a translation of novel cell therapy concepts from research to clinical practice.

Thiol-ene based polymer systems have emerged and gained great popular- ity in material development in general and in biomedical applications specif- ically. The thiol-ene platform is broad and therefore of interest for a variety of applications. At the same time, many aspects of this material platform are largely unexplored, for example material and manufacturing technology developments for microfluidic applications .

In this Ph.D. thesis, thiol-ene materials are explored for use in cell encap- sulation. The marriage of these two technology fields breeds the possibility for a novel microfluidic cell encapsulation approach using a novel encapsulation material. To this end, several new manufacturing technologies for thiol-ene and thiol-ene-epoxy droplet microfluidic devices were developed. Moreover, core-shell microparticle synthesis for cell encapsulation based on a novel co- synthesis concept using a thiol-ene based material was developed and inves- tigated. Finally, a thiol-ene-epoxy system was also used for the formation of microwells and microchannels that improve protein analysis on microarrays.

The first part of the thesis presents the background and state-of-the-art technologies in regards to cell therapy, microfluidics, and thiol-ene based ma- terials. In the second part of the thesis, a novel manufacturing approach of thiol-ene-epoxy material as well as core-shell particle co-synthesis in micro- fluidics using thiol-ene based material are presented and characterized. The third part of the thesis presents the cell viability studies of encapsulated cells using the novel encapsulation material and method. In the final part of the thesis, two applications of thiol-ene-epoxy gaskets for protein detection mi- croarrays are presented. 

Abstract [sv]

Inkapsling av levande celler i mikrokapslar för terapeutiska ändamål är mycket lovande för frmatida behandling av många olika sjukdomar. Emeller- tid är en behandlings effektivitet i hög grad beroende av vilka material som används för inkapsling och vilken teknisk lösning som används för att ska- pa mikrokapslarna. För närvarande återstår det många utmaningar för att omvandla grundforskningresultat till klinisk verklighet, vilken kräver mer än- damålsenliga tillvägagångssätt för att tillverka mikrokapslar i material som är kompatibla med användningsområdena.

De senaste åren har tiol-en baserade polymerer har blivit mycket använda för materialutveckling i stort och för biomedicinska tillämpningar i synnerhet. Med tiol-en kemi kan en mycket stor mängd helt olika syntetiska material framställas, vilket gör tiol-ener intressanta för en mängd applikationer. För närvarande är dock mycket inom denna materialklass outforskat, t.ex. inom material och tillverkningmetodik för mikrofluidiktillämpningar.

I denna avhandling används tiol-ener för cellinkapsling. Sammanslagning av dessa teknologier möjliggör en ny typ av cellinkapsling med nya materi- alegenskaper. En mängd olika tillverkningssätt där tiol-en eller tiol-en-epoxi används för droplet-mikrofluidiksystem utvecklades. Core-shell mikrokapsel- syntes för cell-inkapsling baserat på en ny metod för samtidig syntes av både core och shell utvecklades och karaktäriserades. Slutligen utvecklades ett tiol- en-epoxi system för enkel integrering med proteinmikroarrayer på objektsglas.

I avhandlingens första del presenteras bakgrund och dagens bästa teknolo- gier för terapeutisk cellinkapsling, mikrofluidik och tiol-en baserade material. I avhandlingens andra del presenteras en ny tillverkningsmetod för mikro- strukturerade tiol-en-epoxi artiklar och samtidig syntes av core och shell för mikrokapslar med användande av mikrofluidik. I den tredje delen presenteras cellöverlevandsstudier för de celler som inkapslats med de nya materialen och de nyutvecklade metoderna. I den avslutande delen beskrivs två specifika fall där tiol-en-epoxi komponenter används för proteindetektion och mikroarrayer. 

Place, publisher, year, edition, pages
Stockholm: KTH Royal Institute of Technology, 2017. 137 p.
Series
TRITA-EES, 2017:171
Keyword
Microfluidics, microfabrication, cell encapsulation, core-shell particle, microparticle synthesis, off-stoichiometry-thiol-ene, OSTE, OSTE+, lab-on-a-chip, surface modification, core-shell particle co-synthesis, microar- ray, micromixer, bonding, surface modification, droplet microfluidics, protein screening., Mikrofluidik, mikrofabrikation, cellinkapsling, cores-shell kap- sel, mikrokapselsyntes, lab-on-chip, ickestökiometrisk tiol-en, OSTE, OSTE+, ytmodifiering, samtidig syntes av core-shellkapslar, mikroarray, mikromixer, sammanfogning, dropletmikrofluidik, proteindetektion.
National Category
Engineering and Technology
Research subject
Applied Medical Technology
Identifiers
urn:nbn:se:kth:diva-217995 (URN)978-91-7729-623-2 (ISBN)
Public defence
2017-12-15, M2, Brinellvägen 64, Stockholm, 09:00 (English)
Opponent
Supervisors
Funder
Swedish Childhood Cancer Foundation, 66673
Note

QC 20171122

Available from: 2017-11-22 Created: 2017-11-20 Last updated: 2017-11-22Bibliographically approved

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