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X-ray phase-contrast tomography for high-spatial-resolution zebrafish muscle imaging
KTH, School of Engineering Sciences (SCI), Applied Physics, Biomedical and X-ray Physics.
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2015 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 5Article in journal (Refereed) PublishedText
Abstract [en]

Imaging of muscular structure with cellular or subcellular detail in whole-body animal models is of key importance for understanding muscular disease and assessing interventions. Classical histological methods for high-resolution imaging methods require excision, fixation and staining. Here we show that the three-dimensional muscular structure of unstained whole zebrafish can be imaged with sub-5μm detail with X-ray phase-contrast tomography. Our method relies on a laboratory propagation-based phase-contrast system tailored for detection of low-contrast 4-6μm subcellular myofibrils. The method is demonstrated on 20 days post fertilization zebrafish larvae and comparative histology confirms that we resolve individual myofibrils in the whole-body animal. X-ray imaging of healthy zebrafish show the expected structured muscle pattern while specimen with a dystrophin deficiency (sapje) displays an unstructured pattern, typical of Duchenne muscular dystrophy. The method opens up for whole-body imaging with sub-cellular detail also of other types of soft tissue and in different animal models.

Place, publisher, year, edition, pages
2015. Vol. 5
National Category
Medical Image Processing
Identifiers
URN: urn:nbn:se:kth:diva-181967DOI: 10.1038/srep16625ISI: 000364651700001ScopusID: 2-s2.0-84947067948OAI: oai:DiVA.org:kth-181967DiVA: diva2:902859
Funder
Swedish Research CouncilKnut and Alice Wallenberg Foundation
Note

QC 20160212

Available from: 2016-02-12 Created: 2016-02-11 Last updated: 2016-02-12Bibliographically approved

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Vågberg, WilliamHertz, Hans
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