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Study of protein and RNA in dendritic spines using multi-isotope imaging mass spectrometry (MIMS).
KTH, Centres, Science for Life Laboratory, SciLifeLab. Karolinska Inst, Astrid Lindgren Childrens Hosp, Dept Woman & Child Hlth, Sweden.ORCID iD: 0000-0003-0578-4003
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2014 (English)In: Surface and Interface Analysis, ISSN 0142-2421, E-ISSN 1096-9918, Vol. 46, no Suppl 1Article in journal (Refereed) Published
Abstract [en]

The classical view of neuronal protein synthesis is that proteins are made in the cell body and then transported to their functional sites in the dendrites and the dendritic spines. Indirect evidence, however, suggests that protein synthesis can directly occur in the distal dendrites, far from the cell body. We are developing protocols for dual labeling of RNA and proteins using (15)N-uridine and (18)O- or (13)C-leucine pulse chase in cultured neurons to identify and localize both protein synthesis and fate of newly synthesized proteins. Pilot experiments show discrete localization of both RNA and newly synthesized proteins in dendrites, close to dendritic spines. We have for the first time directly imaged and measured the production of proteins at the subcellular level in the neuronal dendrites, close to the functional sites, the dendritic spines. This will open a powerful way to study neural growth and synapse plasticity in health and disease.

Place, publisher, year, edition, pages
2014. Vol. 46, no Suppl 1
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Cell Biology Biophysics Other Physics Topics
URN: urn:nbn:se:kth:diva-182110DOI: 10.1002/sia.5617ISI: 000345696200040PubMedID: 26379339ScopusID: 2-s2.0-84912091960OAI: diva2:903292

QC 20160307

Available from: 2016-02-15 Created: 2016-02-15 Last updated: 2016-03-07Bibliographically approved

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Brismar, Hjalmar
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